Identification of class 1 and 2 integrons from clinical and environmental Salmonella isolates

被引:18
作者
Lopes Correa, Fabio Ederson [1 ]
da Silva Dantas, Fabiana Gomes [2 ]
Grisolia, Alexeia Barufatti [2 ]
Crispim, Bruno do Amaral [2 ]
Pires Oliveira, Kelly Mari [1 ,2 ]
机构
[1] Fundacao Univ Fed Grande Dourados, Fac Ciencias Saude, BR-79825070 Dourados, MS, Brazil
[2] Fundacao Univ Fed Grande Dourados, Fac Ciencias Biol & Ambientais, BR-79825070 Dourados, MS, Brazil
来源
JOURNAL OF INFECTION IN DEVELOPING COUNTRIES | 2014年 / 8卷 / 12期
关键词
Salmonella; antibiotic resistance; integrons; resistance mechanisms; ANTIMICROBIAL RESISTANCE PROFILE; ANTIBIOTIC USE; SEROVARS; SPREAD;
D O I
10.3855/jidc.4734
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Introduction: The indiscriminate use of antimicrobials has selected for the emergence of resistant strains. Many mechanisms contribute to the spread of antimicrobial-resistant genes, and integrons play a key role in this process. The aim of this study was to describe the serotypes and resistance profiles, and to characterize the presence of integrons in Salmonella strains isolated from Dourados, Mato Grosso do Sul, Brazil. Methodology: Thirty-six isolates from different sources were used. To evaluate the resistance profiles, the determination of minimum inhibitory concentrations together with polymerase chain reaction were used to screen for the presence of class 1 and class 2 integrons. Results: The Infantis serotype of Salmonella was the most frequently isolated serotype. Minimum inhibitory concentrations showed that out of the 36 isolates, 11 (30.5%) were resistant to all the antimicrobials tested. These resistant isolates were separated into three groups: 4 clinical isolates (36.4%), 3 food isolates (36.4%), and 4 water isolates (27.2%). Class 1 integrons occurred in 31 (86.1%) isolates and were found in all 11 resistant isolates (35.5 %) and in 20 (64.5%) of the non-resistant isolates. Class 2 integrons were found in 3 (8.3%) isolates, which were all non-resistant. Conclusion: The presence of an integron did not necessarily confer resistance. Future studies will seek to identify the mechanism behind integron-mediated antimicrobial resistance.
引用
收藏
页码:1518 / 1524
页数:7
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