Platelet-Released Growth Factors Induce Genes Involved in Extracellular Matrix Formation in Human Fibroblasts

被引：9

作者：

Bayer, Andreas ^{[1
]}

Wijaya, Bernard ^{[2
]}

Rademacher, Franziska ^{[2
]}

Moebus, Lena ^{[2
,3
]}

Preuss, Mark

Singh, Michael ^{[1
]}

Tohidnezhad, Mersedeh ^{[4
]}

Kubo, Yusuke ^{[4
]}

Rodewald, Meno ^{[2
]}

Behrendt, Peter ^{[5
]}

Weitkamp, Jan-Tobias ^{[6
]}

Glaeser, Regine ^{[3
]}

Harder, Juergen ^{[3
]}

机构：

[1] Univ Kiel, Inst Anat, D-24098 Kiel, Germany

[2] Univ Med Ctr Schleswig Holstein, Dept Dermatol, Campus Kiel, D-24105 Kiel, Germany

[3] Univ Med Ctr Hamburg Eppendorf, Univ Heart & Vasc Ctr Hamburg, Dept Vasc Med, D-20246 Hamburg, Germany

[4] Rhein Westfal TH Aachen, Dept Anat & Cell Biol, D-52074 Aachen, Germany

[5] Univ Med Ctr Schleswig Holstein, Dept Trauma Surg, Campus Kiel, D-24105 Kiel, Germany

[6] Univ Med Ctr Schleswig Holstein, Dept Oral & Maxillofacial Surg, Campus Kiel, D-24015 Kiel, Germany

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2021年 / 22卷 / 19期

关键词：

platelet-released growth factors (PRGF); wound healing; extracellular matrix (ECM); fibroblasts; TISSUE TRANSGLUTAMINASE; IN-VITRO; GELATINASE-B; PLASMA RICH; FIBRONECTIN; EXPRESSION; MIGRATION; BETA-IG-H3; PROTEIN; PROLIFERATION;

D O I：

10.3390/ijms221910536

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Platelet concentrate products are increasingly used in many medical disciplines due to their regenerative properties. As they contain a variety of chemokines, cytokines, and growth factors, they are used to support the healing of chronic or complicated wounds. To date, underlying cellular mechanisms have been insufficiently investigated. Therefore, we analyzed the influence of Platelet-Released Growth Factors (PRGF) on human dermal fibroblasts. Whole transcriptome sequencing and gene ontology (GO) enrichment analysis of PRGF-treated fibroblasts revealed an induction of several genes involved in the formation of the extracellular matrix (ECM). Real-time PCR analyses of PRGF-treated fibroblasts and skin explants confirmed the induction of ECM-related genes, in particular transforming growth factor beta-induced protein (TGFBI), fibronectin 1 (FN1), matrix metalloproteinase-9 (MMP-9), transglutaminase 2 (TGM2), fermitin family member 1 (FERMT1), collagen type I alpha 1 (COL1A1), a disintegrin and metalloproteinase 19 (ADAM19), serpin family E member 1 (SERPINE1) and lysyl oxidase-like 3 (LOXL3). The induction of these genes was time-dependent and in part influenced by the epidermal growth factor receptor (EGFR). Moreover, PRGF induced migration and proliferation of the fibroblasts. Taken together, the observed effects of PRGF on human fibroblasts may contribute to the underlying mechanisms that support the beneficial wound-healing effects of thrombocyte concentrate products.

引用

页数：16

共 77 条

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