Disease-Specific Alterations in the Enteric Virome in Inflammatory Bowel Disease

被引：957

作者：

Norman, Jason M. ^{[1
]}

Handley, Scott A. ^{[1
]}

Baldridge, Megan T. ^{[1
]}

Droit, Lindsay ^{[1
]}

Liu, Catherine Y. ^{[1
]}

Keller, Brian C. ^{[1
,2
]}

Kambal, Amal ^{[1
]}

Monaco, Cynthia L. ^{[1
,2
]}

Zhao, Guoyan ^{[1
,3
]}

Fleshner, Phillip ^{[4
]}

Stappenbeck, Thaddeus S. ^{[1
]}

McGovern, Dermot P. B. ^{[5
,6
]}

Keshavarzian, Ali ^{[7
]}

Mutlu, Ece A. ^{[7
]}

Sauk, Jenny ^{[8
,9
,10
]}

Gevers, Dirk ^{[11
]}

Xavier, Ramnik J. ^{[8
,9
,10
,11
]}

Wang, David ^{[1
,3
]}

Parkes, Miles ^{[12
,13
]}

Virgin, Herbert W. ^{[1
]}

机构：

[1] Washington Univ, Sch Med, Dept Pathol & Immunol, St Louis, MO 63110 USA

[2] Washington Univ, Sch Med, Dept Med, St Louis, MO 63110 USA

[3] Washington Univ, Sch Med, Dept Mol Microbiol, St Louis, MO 63110 USA

[4] Cedars Sinai Med Ctr, Div Colorectal Surg, Los Angeles, CA 90048 USA

[5] F Widjaja Fdn Inflammatory Bowel & Immunobiol Res, Los Angeles, CA 90048 USA

[6] Cedars Sinai Med Ctr, Los Angeles, CA 90048 USA

[7] Rush Univ, Med Ctr, Div Digest Dis & Nutr, Dept Med, Chicago, IL 60612 USA

[8] Massachusetts Gen Hosp, Gastrointestinal Unit, Boston, MA 02114 USA

[9] Massachusetts Gen Hosp, Ctr Study Inflammatory Bowel Dis, Boston, MA 02114 USA

[10] Harvard Univ, Sch Med, Boston, MA 02114 USA

[11] Broad Inst MIT & Harvard, Cambridge, MA 02142 USA

[12] Univ Cambridge, Addenbrookes Hosp, Div Gastroenterol, Cambridge CB2 0QQ, England

[13] Univ Cambridge, Dept Med, Cambridge CB2 0QQ, England

来源：

CELL | 2015年 / 160卷 / 03期

基金：

美国医疗保健研究与质量局;

关键词：

CROHNS-DISEASE; FECAL MICROBIOTA; GUT MICROBIOTA; METAGENOMIC ANALYSIS; ANTIBIOTICS INDUCE; CYSTIC-FIBROSIS; COMMUNITY; BACTERIOPHAGES; SEQUENCES; EVOLUTION;

D O I：

10.1016/j.cell.2015.01.002

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Decreases in the diversity of enteric bacterial populations are observed in patients with Crohn's disease (CD) and ulcerative colitis (UC). Less is known about the virome in these diseases. We show that the enteric virome is abnormal in CD and UC patients. In-depth analysis of preparations enriched for free virions in the intestine revealed that CD and UC were associated with a significant expansion of Caudovirales bacteriophages. The viromes of CD and UC patients were disease and cohort specific. Importantly, it did not appear that expansion and diversification of the enteric virome was secondary to changes in bacterial populations. These data support a model in which changes in the virome may contribute to intestinal inflammation and bacterial dysbiosis. We conclude that the virome is a candidate for contributing to, or being a biomarker for, human inflammatory bowel disease and speculate that the enteric virome may play a role in other diseases.

引用

页码：447 / 460

页数：14

共 66 条

[1] Temporal Bacterial Community Dynamics Vary Among Ulcerative Colitis Patients After Fecal Microbiota Transplantation [J].

Angelberger, Sieglinde ;

Reinisch, Walter ;

Makristathis, Athanasios ;

Lichtenberger, Cornelia ;

Dejaco, Clemens ;

Papay, Pavol ;

Novacek, Gottfried ;

Trauner, Michael ;

Loy, Alexander ;

Erry, David B. .

AMERICAN JOURNAL OF GASTROENTEROLOGY, 2013, 108 (10) :1620-1630

[2] Bacteriophage adhering to mucus provide a non-host-derived immunity [J].

Barr, Jeremy J. ;

Auro, Rita ;

Furlan, Mike ;

Whiteson, Katrine L. ;

Erb, Marcella L. ;

Pogliano, Joe ;

Stotland, Aleksandr ;

Wolkowicz, Roland ;

Cutting, Andrew S. ;

Doran, Kelly S. ;

Salamon, Peter ;

Youle, Merry ;

Rohwer, Forest .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2013, 110 (26) :10771-10776

[3] Norovirus Triggered Microbiota-driven Mucosal Inflammation in Interleukin 10-deficient Mice [J].

Basic, Marijana ;

Keubler, Lydia M. ;

Buettner, Manuela ;

Achard, Marcel ;

Breves, Gerhard ;

Schroeder, Bernd ;

Smoczek, Anna ;

Joerns, Anne ;

Wedekind, Dirk ;

Zschemisch, Nils H. ;

Guenther, Claudia ;

Neumann, Detlef ;

Lienenklaus, Stefan ;

Weiss, Siegfried ;

Hornef, Mathias W. ;

Maehler, Michael ;

Bleich, Andre .

INFLAMMATORY BOWEL DISEASES, 2014, 20 (03) :431-443

[4] Role of the Microbiota in Immunity and Inflammation [J].

Belkaid, Yasmine ;

Hand, Timothy W. .

CELL, 2014, 157 (01) :121-141

[5] Metagenomic analyses of an uncultured viral community from human feces [J].

Breitbart, M ;

Hewson, I ;

Felts, B ;

Mahaffy, JM ;

Nulton, J ;

Salamon, P ;

Rohwer, F .

JOURNAL OF BACTERIOLOGY, 2003, 185 (20) :6220-6223

[6] Phages and the evolution of bacterial pathogens:: From genomic rearrangements to lysogenic conversion [J].

Brüssow, H ;

Canchaya, C ;

Hardt, WD .

MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 2004, 68 (03) :560-+

[7] Probiotics for induction of remission in Crohn's disease [J].

Butterworth, Andrew D. ;

Thomas, Adrian G. ;

Akobeng, Anthony Kwaku .

COCHRANE DATABASE OF SYSTEMATIC REVIEWS, 2008, (03)

[8] Virus-Plus-Susceptibility Gene Interaction Determines Crohn's Disease Gene Atg16L1 Phenotypes in Intestine [J].

Cadwell, Ken ;

Patel, Khushbu K. ;

Maloney, Nicole S. ;

Liu, Ta-Chiang ;

Ng, Aylwin C. Y. ;

Storer, Chad E. ;

Head, Richard D. ;

Xavier, Ramnik ;

Stappenbeck, Thaddeus S. ;

Virgin, Herbert W. .

CELL, 2010, 141 (07) :1135-U64

[9] Global patterns of 16S rRNA diversity at a depth of millions of sequences per sample [J].

Caporaso, J. Gregory ;

Lauber, Christian L. ;

Walters, William A. ;

Berg-Lyons, Donna ;

Lozupone, Catherine A. ;

Turnbaugh, Peter J. ;

Fierer, Noah ;

Knight, Rob .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2011, 108 :4516-4522

[10] QIIME allows analysis of high-throughput community sequencing data [J].

Caporaso, J. Gregory ;

Kuczynski, Justin ;

Stombaugh, Jesse ;

Bittinger, Kyle ;

Bushman, Frederic D. ;

Costello, Elizabeth K. ;

Fierer, Noah ;

Pena, Antonio Gonzalez ;

Goodrich, Julia K. ;

Gordon, Jeffrey I. ;

Huttley, Gavin A. ;

Kelley, Scott T. ;

Knights, Dan ;

Koenig, Jeremy E. ;

Ley, Ruth E. ;

Lozupone, Catherine A. ;

McDonald, Daniel ;

Muegge, Brian D. ;

Pirrung, Meg ;

Reeder, Jens ;

Sevinsky, Joel R. ;

Tumbaugh, Peter J. ;

Walters, William A. ;

Widmann, Jeremy ;

Yatsunenko, Tanya ;

Zaneveld, Jesse ;

Knight, Rob .

NATURE METHODS, 2010, 7 (05) :335-336

← 1 2 3 4 5 6 7 →