On the relationship of neuropeptide Y Y1 receptor-immunoreactive neuronal structures to the neuropeptide Y-immunoreactive nerve terminal networks. A double immunolabelling analysis in the rat brain

被引:27
作者
Caberlotto, L
Tinner, B
Bunnemann, B
Agnati, L
Fuxe, K [1 ]
机构
[1] Karolinska Inst, Div Cellular & Mol Neurochem, Dept Neurosci, S-17175 Stockholm, Sweden
[2] Karolinska Hosp, Psychiat Sect, Dept Clin Neurosci, S-17176 Stockholm, Sweden
[3] Glaxo Wellcome SPA, Med Res Ctr, I-37100 Verona, Italy
[4] Univ Modena, Dept Human Physiol, I-41100 Modena, Italy
关键词
rat brain; immunohistochemistry; NPY; NPY Y1 receptor; volume transmission;
D O I
10.1016/S0306-4522(98)00089-X
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Neuropeptide Y is the most abundant peptide in the mammalian central nervous system and exhibits a variety of potent neurobiological functions. In the present study, double immunolabelling histochemistry was performed, using previously characterized antibodies against neuropeptide Y and the neuropeptide Y Y1 receptor subtype, to clarify the cellular distribution of Y1 receptors in the rat brain in relation to the neuropeptide Y-immunoreactive systems. Based on fluoresence and confocal laser microscopy analysis, morphological evidence is presented that the perikaryal and dendritic Y1 receptorlike immunoreactivity demonstrated in discrete regions of the tel-, diencephalon and of the lower brain stem, shown to be cytoplasmic and membrane associated, in many brain regions is not co-distributed with the neuropeptide Y-immunoreactive terminal network. These findings may partly be explained by the existence of volume transmission in Y1 receptor-mediated neuropeptide Y transmission involving short to long distance diffusion and/or convection of neuropeptide Y from its site of release to the neuronal target cells, containing the high-affinity Y1 receptors. Furthermore, neuropeptide Y and Y1 receptor-like immunoreactivities were in no case co-localized in the same nerve cell, suggesting that, in the rat brain, the Y1 receptor subtype may not be a neuropeptide Y autoreceptor. (C) 1998 IBRO. Published by Elsevier Science Ltd.
引用
收藏
页码:827 / 845
页数:19
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