Protection of multiple antioxidants against heme protein oxidation and lipid peroxidation induced by CBrCl3 in liver, lung, kidney, heart, and spleen

Protection of multiple antioxidants against heme protein oxidation and lipid peroxidation induced by bromotrichloromethane (CBrCl3) was studied in a tissue homogenate system. Rats were fed a basal diet (vitamin E and selenium deficient) supplemented with different kinds and amounts of antioxidants. Diet 1 was supplemented with vitamin E and selenium (control diet). Diet 2 was supplemented with vitamin E, selenium, and the water-soluble/less fat-soluble antioxidants coenzyme Q(0), trolox c, and acetylcysteine. Diet 3 was supplemented with vitamin E, selenium, and both water- and fat-soluble antioxidants trolox c, coenzyme Q(0), acetylcysteine, beta-carotene, ascorbic acid B-palmitate, coenzyme Q(10), and canthaxanthin. (+)-Catechin was added to diets 2 and 3 as a representative flavonoid. In general, lower concentrations of thiobarbituric acid reactive substances and oxidized heme proteins were found in tissues hom rats fed diets 2 and 3 than from rats fed diet 1, suggesting that increased quantity and diversity of antioxidants in diets significantly strengthen protection against oxidative damage to tissues.