Molecular Orientational Order Probed by Coherent Anti-Stokes Raman Scattering (CARS) and Stimulated Raman Scattering (SRS) Microscopy: A Spectral Comparative Study

被引：28

作者：

Duboisset, Julien ^{[1
]}

Berto, Pascal ^{[1
]}

Gasecka, Paulina ^{[1
]}

Bioud, Fatma-Zohra ^{[1
]}

Ferrand, Patrick ^{[1
]}

Rigneault, Herve ^{[1
]}

Brasselet, Sophie ^{[1
]}

机构：

[1] Aix Marseille Univ, CNRS, Cent Marseille, Inst Fresnel,UMR 7249, F-13013 Marseille, France

来源：

JOURNAL OF PHYSICAL CHEMISTRY B | 2015年 / 119卷 / 07期

关键词：

IN-VIVO; SENSITIVITY; GENERATION; TISSUES; FIBERS; TENDON; MYELIN;

D O I：

10.1021/jp5113813

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

We investigate how to;extract information on the orientational order of molecular bonds in biological samples from penalized coherent anti Stokes Raman, scattering (CARS) and stimulated Raman scattering (SRS) microscopy. Experimentally; the Mean orientation of the molecular angular distribution, as well as its second and fourth orders of Symmetry, are estimated by monitoring intensity signals Under a Varying incident polarization. We provide a generic method of analysis of polarized Signals in both CARS and SRS contrasts, and apply it to imaging Of lipid bonds orientational order in multilamellar vesicles A comparison of the two contrasts in the lipid region around 3000 cm(-1) shows that while SRS allows retrieving pure molecular order information, CARS is generally tainted by a bias from the nonresonant contribution.

引用

页码：3242 / 3249

页数：8

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