C. butyricum lipoteichoic acid inhibits the inflammatory response and apoptosis in HT-29 cells induced by S. aureus lipoteichoic acid

Lipoteichoic acid (LTA) is one of microbe-associated molecular pattern (MAMP) molecules of gram positive bacteria. In this study, we demonstrated that Clostridium butyricum LTA (bLTA) significantly inhibited the inflammatory response and apoptosis induced by Staphylococcus aureus LTA (aLTA) in HT-29 cells. aLTA stimulated the inflammatory responses by activating a strong signal transduction cascade through NF-kappa B and ERK, but bLTA did not activate the signaling pathway. bLTA pretreatment inhibited the activation of the NF-kappa B and ERK signaling pathway induced by aLTA. The expression and release of cytokines such as IL-8 and TNF-alpha, were also suppressed by bLTA pretreatment. aLTA treatment induced apoptosis in HT-29 cells, but bLTA did not affect the viability of the cells. Further study indicated that bLTA inhibited apoptosis in HT-29 cells induced by aLTA. These results suggest that bLTA may act as an aLTA antagonist and that an antagonistic bLTA may be a useful agent for suppressing the pro-inflammatory activities of gram-positive pathogenic bacteria. (C) 2016 Elsevier B.V. All rights reserved.