Down-regulated lncRNA MEG3 promotes osteogenic differentiation of human dental follicle stem cells by epigenetically regulating Wnt pathway

被引：75
作者：
Deng, Lidi [1 ]
Hong, Hong [2 ]
Zhang, Xueqin [1 ]
Chen, Dongru [3 ]
Chen, Zhengyuan [1 ]
Ling, Junqi [4 ]
Wu, Liping [1 ]
机构：
[1] Sun Yat Sen Univ, Guangdong Prov Key Lab Stomatol, Hosp Stomatol, Guanghua Sch Stomatol,Dept Orthodont, Guangzhou 510055, Guangdong, Peoples R China
[2] Sun Yat Sen Univ, Guangdong Prov Key Lab Stomatol, Hosp Stomatol, Guanghua Sch Stomatol,Zhujiang New Town Dent Clin, Guangzhou 510055, Guangdong, Peoples R China
[3] Sun Yat Sen Univ, Guangdong Prov Key Lab Stomatol, Hosp Stomatol, Guanghua Sch Stomatol,Dept Prevent Dent, Guangzhou 510055, Guangdong, Peoples R China
[4] Sun Yat Sen Univ, Guangdong Prov Key Lab Stomatol, Hosp Stomatol, Guanghua Sch Stomatol,Dept Endodont, Guangzhou 510055, Guangdong, Peoples R China
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2018年 / 503卷 / 03期
基金：
中国国家自然科学基金;
关键词：
LncRNA MEG3; Osteogenic differentiation; Human dental follicle stem cells; Epigenetics; Wnt pathway; SIGNALING PATHWAY; DNA METHYLATION; EZH2; GENES; TRANSCRIPTION; PPAR;
D O I：
10.1016/j.bbrc.2018.07.160
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Our previous long noncoding RNA (IncRNA) microarray results showed that IncRNA MEG3 (maternally expressed 3) was significantly downregulated in human dental follicle cells than human periodontal ligament cells. Latest studies show that MEG3 contributes to polycomb repressive complex 2 (PRC2) recruitment to silence gene expression. The enhancer of zeste homolog 2 (EZH2), a crucial catalytic subunit of PRC2, mediates gene silencing and participates in cell lineage determination via methyltransferase activity. In this study, we found that the expression of EZH2 and H3K27me3 (trimethylation on lysine 27 in histone H3) decreased during osteogenesis of human dental follicle stem cells (hDFSCs). Knockdown studies of MEG3 and EZH2 by siRNA showed that MEG3/EZH2 negatively regulated osteogenesis of hDFSCs. We investigated the role of Wnt signaling pathway during the osteogenesis of hDFSCs and its relationship with EZH2. Besides, we studied the key genes of the canonical/noncanonical Wnt signaling pathway which might be related to EZH2. ChIP (chromatin immunoprecipitation) analysis showed that these effects were due to the EZH2 regulation of H3K27me3 level on the Wnt genes pro motors. We first demonstrated that the decrease of MEG3 or EZH2 activated the Wnt/beta-catenin signaling pathway via epigenetically regulating the H3K27me3 level on the Wnt genes promotors. Our research offers a new target for periodontal tissue engineering and osteogenic tissue regeneration. (C) 2018 Elsevier Inc. All rights reserved.
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收藏
页码：2061 / 2067
页数：7
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