Molecular cloning and expression analysis of feline α1-microglobulin

被引：3

作者：

Nakata, Junya ^{[1
]}

Nakahari, Aiko ^{[1
]}

Kato, Yohei ^{[1
]}

Yamachi, Nanako ^{[1
]}

Sato, Nami ^{[1
]}

Yonezawa, Tomohiro ^{[1
]}

Takahashi, Chika ^{[1
]}

Chikazawa, Seishiro ^{[1
]}

Hori, Yasutomo ^{[1
]}

Kanai, Kazutaka ^{[1
]}

Ito, Naoyuki ^{[1
]}

Higuchi, Seiichi ^{[1
]}

Hoshi, Fumio ^{[1
]}

机构：

[1] Kitasato Univ, Sch Vet Med & Anim Sci, Dept Small Anim Internal Med, Towada, Aomori 034, Japan

来源：

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 2011年 / 139卷 / 01期

关键词：

alpha(1)-Microglobulin; Cat; Cloning; Expression; TISSUE-SPECIFIC EXPRESSION; LIPOCALIN PROTEIN FAMILY; MESSENGER-RNA; ALPHA-1-MICROGLOBULIN; CDNA; HC; MICROGLOBULIN; BIKUNIN; COMPLEX; URINE;

D O I：

10.1016/j.vetimm.2010.08.002

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Full-length cDNA that encodes feline alpha(1)-microglobulin (Fe alpha(1) m)-bikunin was obtained from a feline liver and cloned using an oligo-capping method. The Fe alpha(1) m-bikunin cDNA was found to contain 1284 nucleotides, and Fe alpha(1) m was found to include an open reading frame encoding a polypeptide of 201 amino acids. The deduced amino acid sequence of Fe alpha(1) m showed varying amino acid identity when compared with the published sequences of the related alpha(1)-m of other species, ranging from 71.1 to 82.1%. Fe alpha(1) m mRNA expression was confirmed by reverse transcription polymerase chain reaction (RT-PCR) and real-time PCR analysis in the cerebrum, cerebellum, lung, heart, liver, spleen, pancreas, kidney, adrenal gland, and testicle. The highest Fe alpha(1) m mRNA level was found in the liver. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：79 / 82

页数：4

共 18 条

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