Myristic Acid Supplementation Aggravates High Fat Diet-Induced Adipose Inflammation and Systemic Insulin Resistance in Mice

被引:21
作者
Saraswathi, Viswanathan [1 ,2 ]
Kumar, Narendra [1 ,2 ]
Ai, Weilun [1 ,2 ]
Gopal, Thiyagarajan [1 ,2 ]
Bhatt, Saumya [1 ,2 ]
Harris, Edward N. [3 ]
Talmon, Geoffrey A. [4 ]
Desouza, Cyrus, V [1 ,2 ]
机构
[1] Univ Nebraska Med Ctr, Dept Internal Med, Div Diabet Endocrinol & Metab, Omaha, NE 68198 USA
[2] VA Nebraska Western Iowa Hlth Care Syst, Res Serv, Omaha, NE 68105 USA
[3] Univ Nebraska, Dept Biochem, Lincoln, NE 68588 USA
[4] Univ Nebraska Med Ctr, Dept Pathol & Microbiol, Omaha, NE 68198 USA
关键词
myristic acid; saturated fatty acids; obesity; insulin resistance; adipose inflammation; PALMITIC ACID; CHOLESTEROL LEVELS; OBESITY; IMPROVES; GLUCOSE; TRIGLYCERIDES; LIPOPROTEINS; METABOLISM; LIPIDS; LDL;
D O I
10.3390/biom12060739
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Saturated fatty acids (SFAs) are considered to be detrimental to human health. One of the SFAs, myristic acid (MA), is known to exert a hypercholesterolemic effect in mice as well as humans. However, its effects on altering adipose tissue (AT) inflammation and systemic insulin resistance (IR) in obesity are still unclear. Here, we sought to determine the effects of a high fat (HF) diet supplemented with MA on obesity-associated metabolic disorders in mice. Wild-type C57BL/6 mice were fed a HF diet in the presence or absence of 3% MA for 12 weeks. Plasma lipids, plasma adipokines, AT inflammation, systemic IR, glucose homeostasis, and hepatic steatosis were assessed. The body weight and visceral adipose tissue (VAT) mass were significantly higher in mice receiving the HF+MA diet compared to HF diet-fed controls. Plasma total cholesterol levels were marginally increased in HF+MA-fed mice compared to controls. Fasting blood glucose was comparable between HF and HF+MA-fed mice. Interestingly, the plasma insulin and HOMA-IR index, a measure of insulin resistance, were significantly higher in HF+MA-fed mice compared to HF controls. Macrophage and inflammatory markers were significantly elevated in the AT and AT-derived stromal vascular cells upon MA feeding. Moreover, the level of circulating resistin, an adipokine promoting insulin resistance, was significantly higher in HF+MA-fed mice compared with HF controls. The insulin tolerance test revealed that the IR was higher in mice receiving the MA supplementation compared to HF controls. Moreover, the glucose tolerance test showed impairment in systemic glucose homeostasis in MA-fed mice. Analyses of liver samples showed a trend towards an increase in liver TG upon MA feeding. However, markers of oxidative stress and inflammation were reduced in the liver of mice fed an MA diet compared to controls. Taken together, our data suggest that chronic administration of MA in diet exacerbates obesity-associated insulin resistance and this effect is mediated in part, via increased AT inflammation and increased secretion of resistin.
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页数:15
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共 51 条
[1]   Fatty Acid Content of Plasma Triglycerides May Contribute to the Heterogeneity in the Relationship Between Abdominal Obesity and the Metabolic Syndrome [J].
Aristizabal, Juan C. ;
Barona, Jacqueline ;
Gonzalez-Zapata, Laura I. ;
Deossa, Gloria C. ;
Estrada, Alejandro .
METABOLIC SYNDROME AND RELATED DISORDERS, 2016, 14 (06) :311-317
[2]   The effects of changing dairy intake on trans and saturated fatty acid levels- results from a randomized controlled study [J].
Benatar J.R. ;
Stewart R.A. .
Nutrition Journal, 13 (1)
[3]   Palmitic Acid: Physiological Role, Metabolism and Nutritional Implications [J].
Carta, Gianfranca ;
Murru, Elisabetta ;
Banni, Sebastiano ;
Manca, Claudia .
FRONTIERS IN PHYSIOLOGY, 2017, 8
[4]   Metabolism dysregulation induces a specific lipid signature of nonalcoholic steatohepatitis in patients [J].
Chiappini, Franck ;
Coilly, Audrey ;
Kadar, Hanane ;
Gual, Philippe ;
Tran, Albert ;
Desterke, Christophe ;
Samuel, Didier ;
Duclos-Vallee, Jean-Charles ;
Touboul, David ;
Bertrand-Michel, Justine ;
Brunelle, Alain ;
Guettier, Catherine ;
Le Naour, Francois .
SCIENTIFIC REPORTS, 2017, 7
[5]   Hepatic and serum lipid signatures specific to nonalcoholic steatohepatitis in murine models [J].
Chiappini, Franck ;
Desterke, Christophe ;
Bertrand-Michel, Justine ;
Guettier, Catherine ;
Le Naour, Francois .
SCIENTIFIC REPORTS, 2016, 6
[6]   Individual saturated fatty acids are associated with different components of insulin resistance and glucose metabolism: the GOCADAN study [J].
Ebbesson, Sven O. E. ;
Tejero, M. Elizabeth ;
Lopez-Alvarenga, Juan Carlos ;
Harris, William S. ;
Ebbesson, Lars O. E. ;
Devereux, Richard B. ;
MacCluer, Jean W. ;
Wenger, Charlotte ;
Laston, Sandra ;
Fabsitz, Richard R. ;
Howard, Barbara V. ;
Comuzzie, Anthony G. .
INTERNATIONAL JOURNAL OF CIRCUMPOLAR HEALTH, 2010, 69 (04) :344-351
[7]   Palmitic acid is an intracellular signaling molecule involved in disease development [J].
Fatima, Sarwat ;
Hu, Xianjing ;
Gong, Rui-Hong ;
Huang, Chunhua ;
Chen, Minting ;
Wong, Hoi Leong Xavier ;
Bian, Zhaoxiang ;
Kwan, Hiu Yee .
CELLULAR AND MOLECULAR LIFE SCIENCES, 2019, 76 (13) :2547-2557
[8]   Saturated fatty acids and insulin resistance [J].
Funaki, Makoto .
JOURNAL OF MEDICAL INVESTIGATION, 2009, 56 (3-4) :88-92
[9]   Nanoformulated SOD1 ameliorates the combined NASH and alcohol-associated liver disease partly via regulating CYP2E1 expression in adipose tissue and liver [J].
Gopal, Thiyagarajan ;
Kumar, Narendra ;
Perriotte-Olson, Curtis ;
Casey, Carol A. ;
Donohue, Terrence M., Jr. ;
Harris, Edward N. ;
Talmon, Geoffrey ;
Kabanov, Alexander, V ;
Saraswathi, Viswanathan .
AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 2020, 318 (03) :G428-G438
[10]   Palmitoleate Induces Hepatic Steatosis but Suppresses Liver Inflammatory Response in Mice [J].
Guo, Xin ;
Li, Honggui ;
Xu, Hang ;
Halim, Vera ;
Zhang, Weiyu ;
Wang, Huan ;
Ong, Kuok Teong ;
Woo, Shih-Lung ;
Walzem, Rosemary L. ;
Mashek, Douglas G. ;
Dong, Hui ;
Lu, Fuer ;
Wei, Lai ;
Huo, Yuqing ;
Wu, Chaodong .
PLOS ONE, 2012, 7 (06)