Sample Preparation for Determination of Neomycin in Swine Tissues by Liquid Chromatography-Fluorescence Detection

被引：16

作者：

Chen, Yiqiang ^{[1
]}

Hu, Xiuzhi ^{[2
]}

Xiao, Xilong ^{[1
]}

机构：

[1] China Agr Univ, Natl Vet Drug Safety Evaluat Ctr, Beijing 100193, Peoples R China

[2] Heibei Univ Engn, Handan, Hebei, Peoples R China

来源：

ANALYTICAL LETTERS | 2010年 / 43卷 / 16期

关键词：

9-Fluorenylmethyl chloroformate; Liquid chromatography; Neomycin; Swine tissue; TANDEM-MASS-SPECTROMETRY; SOLID-PHASE EXTRACTION; AMINOGLYCOSIDES; GENTAMICIN; KIDNEY; ASSAY; MILK;

D O I：

10.1080/00032711003725599

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A liquid chromatography method was developed for the determination of neomycin in swine tissues. Sample clean-up was especially focused on in this research. After neomycin was extracted from tissue with 10% trichloroacetic acid (w/v) solution, several clean-up procedures using MCX, CBA, WCX, and C18 solid-phase extraction (SPE) columns were compared for the purification effects and recoveries. The best result was obtained using MCX SPE column. The purified extract was derivatized with 9-fluorenylmethyl chloroformate and then separated on a C18 column and detected by a fluorescence detector. The spiked recoveries at 0.2-10mg/kg ranged from 80.8-95.3% with coefficient of variations less than 12.1%.

引用

页码：2496 / 2504

页数：9

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