Oligonucleotide aptamers: Recent advances in their screening, molecular conformation and therapeutic applications

被引：62

作者：

Xiao, Xueran ^{[1
]}

Li, Hui ^{[2
]}

Zhao, Lijian ^{[1
]}

Zhang, Yanfen ^{[3
]}

Liu, Zhongcheng ^{[1
]}

机构：

[1] Hebei Univ, Coll Pharmaceut Sci, Inst Life Sci & Green Dev, Key Lab Pharmaceut Qual Control Hebei Prov, Baoding 071002, Peoples R China

[2] Peking Univ, Dept Urol, Int Hosp, Beijing 102206, Peoples R China

[3] Hebei Univ, Technol Transfer Ctr, Baoding 071002, Peoples R China

来源：

BIOMEDICINE & PHARMACOTHERAPY | 2021年 / 143卷

关键词：

Aptamer; SELEX; Screening method; Nucleic acid drug; Clinical trials; IN-VITRO SELECTION; STRANDED-DNA APTAMERS; G-QUADRUPLEX APTAMER; CAPILLARY-ELECTROPHORESIS; HIGH-AFFINITY; CAPTURE-SELEX; EQUILIBRIUM MIXTURES; SYSTEMATIC EVOLUTION; RECEPTOR-BINDING; RNA APTAMERS;

D O I：

10.1016/j.biopha.2021.112232

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Aptamers are single stranded oligonucleotides with specific recognition and binding ability to target molecules, which can be obtained by Systematic Evolution of Ligands by Exponential Enrichment (SELEX). Aptamers have the advantages of low molecular weight, low immunogenicity, easy modification and high stability. They play promising role in promoting food safety, monitoring the environment and basic research, especially in clinical diagnosis and therapeutic drugs. To date, great achievements regarding the selection, modifications and application of aptamers have been made. However, since it is still a challenge to obtain aptamers with high affinity in a more effective way, few aptamer-based products have already successfully entered into clinical use. This review aims to provide a thorough overview of the latest advances in this rapidly developing field, focusing on aptamer screening methods for different targets, the structure of the interaction between aptamers and target substances, and the challenges and potential of current therapeutic aptamers.

引用

页数：14

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