Involvement of ERK1/2 signalling and growth-related molecules' expression in response to heat stress-induced damage in rat jejunum and IEC-6 cells

被引:48
作者
Yu, Jin [1 ,2 ]
Yin, Peng [2 ]
Yin, Jingdong [2 ]
Liu, Fenghua [1 ,2 ,3 ]
Zhu, Xiaoyu [2 ,3 ]
Cheng, Guiling [1 ,2 ,3 ]
Guo, Kaijun [1 ]
Yin, Yulong [4 ]
Xu, Jianqin [2 ,3 ]
机构
[1] China Agr Univ, Dept Anim Sci & Technol, Beijing 100094, Peoples R China
[2] China Agr Univ, CAU BUA TCVM Teaching & Res Team, Coll Vet Med, Beijing 100094, Peoples R China
[3] China Agr Univ, Beijing Key Lab TCVM, Beijing 100094, Peoples R China
[4] Inst Subtrop Agr, Key Lab Agroecol Proc Subtrop Reg, Changsha, Hunan, Peoples R China
基金
北京市自然科学基金; 中国国家自然科学基金;
关键词
ERK1/2; growth-related molecules; heat stress; IEC-6; Cell; microarray; rat; small intestine; WHOLE-BODY HYPERTHERMIA; LYMPHOMA U937 CELLS; GENE-EXPRESSION; INDUCED APOPTOSIS; OXIDATIVE STRESS; DNA-DAMAGE; BLOOD-FLOW; DIFFERENTIATION; TRANSDUCTION; MECHANISMS;
D O I
10.3109/02656736.2010.481276
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Our previous studies found small intestine epithelial tissues from several different animals (including rats, pigs and chickens) became significantly damaged following exposure to extreme heat. However, damaged tissue was rapidly repaired or regenerated in the following few days. Growth-related molecules are critical for cellular survival and promote endothelial cell proliferation and migration. The ERK1/2 signalling pathway is reported to regulate the growth and adaptation of endothelial cells to both physiological and pathological stimuli. However, little information is available concerning both growth-related molecules and ERK1/2 in response to heat stress. Herein, we employed both live rats and rat IEC-6 cells to investigate growth-related molecule expression and ERK1/2 activation in heat stress. Heat stress caused significant morphological damage to rat intestinal tissue and IEC-6 cells, reduced cell growth and proliferation, induced apoptosis, altered growth-related molecule mRNA expression and increased ERK1/2 phosphorylation. Addition of U0126 (a selective inhibitor of MEK kinase responsible for ERK phosphorylation) combined with heat stress exacerbated the morphological damage and apoptosis. With the addition of U0126, further up-or down-regulation of Egfr, Ctgf, Tgif, Vegfa, Okl38 and Gdf15 in response to heat stress was observed. In conclusion, extreme heat stress caused obvious damage to rat jejunum and IEC-6 cells. Both growth-related molecule expression and ERK1/2 phosphorylation were involved in response to heat stress. ERK1/2 inhibition exacerbated apoptosis and affected growth factor mRNA expression in heat stress.
引用
收藏
页码:538 / 555
页数:18
相关论文
共 36 条
  • [1] Epidermal growth factor and betacellulin mediate signal transduction through co-expressed ErbB2 and ErbB3 receptors
    Alimandi, M
    Wang, LM
    Bottaro, D
    Lee, CC
    Kuo, A
    Frankel, M
    Fedi, P
    Tang, C
    Lippman, M
    Pierce, JH
    [J]. EMBO JOURNAL, 1997, 16 (18) : 5608 - 5617
  • [2] Oral trefoil peptides protect against ethanol- and indomethacin-induced gastric injury in rats
    Babyatsky, MW
    deBeaumont, M
    Thim, L
    Podolsky, DK
    [J]. GASTROENTEROLOGY, 1996, 110 (02) : 489 - 497
  • [3] Global gene expression analyses reveal changes in biological processes after hyperthermia in a rat glioma model
    Borkamo, Erling Dahl
    Dahl, Olav
    Bruland, Ove
    Fluge, Oystein
    [J]. INTERNATIONAL JOURNAL OF HYPERTHERMIA, 2008, 24 (05) : 425 - 441
  • [4] Characterization of the rat, mouse, and human genes of growth/differentiation factor-15/macrophage inhibiting cytokine-1 (GDF-15/MIC-1)
    Böttner, M
    Laaff, M
    Schechinger, B
    Rappold, G
    Unsicker, K
    Suter-Crazzolara, C
    [J]. GENE, 1999, 237 (01) : 105 - 111
  • [5] CHEUNG EC, 2004, SCI STKE, pE45
  • [6] CONCORDET JP, 1993, AM J PHYSIOL, V265, P626
  • [7] Changes in hepatic blood flow during whole body hyperthermia
    Deja, Maria
    Ahlers, Olaf
    Macguill, Martin
    Wust, Peter
    Hildebrandt, Bert
    Riess, Hanno
    Kerner, Thoralf
    [J]. INTERNATIONAL JOURNAL OF HYPERTHERMIA, 2010, 26 (02) : 95 - 100
  • [8] Glucagon-like peptides: Regulators of cell proliferation, differentiation, and apoptosis
    Drucker, DJ
    [J]. MOLECULAR ENDOCRINOLOGY, 2003, 17 (02) : 161 - 171
  • [9] Foncea R, 2000, BIOL RES, V33, P89
  • [10] Growth factors, cytokines and their receptors as downstream targets of arylhydrocarbon receptor (AhR) signaling pathways
    Haarmann-Stemmann, Thomas
    Bothe, Hanno
    Abel, Josef
    [J]. BIOCHEMICAL PHARMACOLOGY, 2009, 77 (04) : 508 - 520