Descemet's Membrane Supports Corneal Endothelial Cell Regeneration in Rabbits

被引：35

作者：

Chen, Jingyao ^{[1
,3
,4
]}

Li, Zhiyuan ^{[1
,3
,5
]}

Zhang, Liying ^{[1
,3
]}

Ou, Shangkun ^{[1
,2
,3
]}

Wang, Yanzi ^{[1
,3
]}

He, Xin ^{[1
,2
,3
]}

Zou, Dulei ^{[1
,3
,7
]}

Jia, Changkai ^{[1
,3
]}

Hu, Qianqian ^{[1
,3
]}

Yang, Shu ^{[1
,3
]}

Li, Xian ^{[1
,3
]}

Li, Juan ^{[1
,3
]}

Wang, Junqi ^{[1
,3
]}

Sun, Huimin ^{[1
,3
]}

Chen, Yongxiong ^{[1
,3
]}

Zhu, Ying-Ting ^{[6
]}

Tseng, Scheffer C. G. ^{[6
]}

Liu, Zuguo ^{[1
,2
,3
]}

Li, Wei ^{[1
,2
,3
]}

机构：

[1] Xiamen Univ, Coll Med, Inst Eye, Xiamen, Fujian, Peoples R China

[2] Xiamen Univ, Affiliated Xiamen Eye Ctr, Xiamen, Fujian, Peoples R China

[3] Fujian Prov Key Lab Ophthalmol & Visual Sci, Xiamen, Fujian, Peoples R China

[4] Kunming Med Univ, Yanan Hosp, Kunming, Yunnan, Peoples R China

[5] Southern Med Univ Chenzhou, Affiliated Hosp, Chenzhou, Hunan, Peoples R China

[6] Ocular Surface Ctr, Miami, FL USA

[7] Shandong Acad Med Sci, Shandong Eye Hosp, Shandong Eye Inst, Jinan, Shandong, Peoples R China

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

基金：

中国国家自然科学基金;

关键词：

SMOOTH MUSCLE ACTIN; MYOFIBROBLAST TRANSFORMATION; RETROCORNEAL MEMBRANE; EXPRESSION; COLLAGEN; CLASSIFICATION; COMPLICATIONS; KERATOPLASTY; DETACHMENT; FREEZE;

D O I：

10.1038/s41598-017-07557-2

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Descemet's membrane (DM) helps maintain phenotype and function of corneal endothelial cells under physiological conditions, while little is known about the function of DM in corneal endothelial wound healing process. In the current study, we performed in vivo rabbit corneal endothelial cell (CEC) injury via CEC scraping, in which DM remained intact after CECs removal, or via DM stripping, in which DM was removed together with CECs. We found rabbit corneas in the CEC scraping group healed with transparency restoration, while there was posterior fibrosis tissue formation in the corneas after DM stripping on day 14. Following CEC scraping on day 3, cells that had migrated toward the central cornea underwent a transient fibrotic endothelial-mesenchymal transition (EMT) which was reversed back to an endothelial phenotype on day 14. However, in the corneas injured via DM stripping, most of the cells in the posterior fibrosis tissue did not originate from the corneal endothelium, and they maintained fibroblastic phenotype on day 14. We concluded that corneal endothelial wound healing in rabbits has different outcomes depending upon the presence or absence of Descemet's membrane. Descemet's membrane supports corneal endothelial cell regeneration in rabbits after endothelial injury.

引用

页数：13

共 46 条

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[2]

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[3]

BROWN SI, 1966, ARCH OPHTHALMOL-CHIC, V75, P518

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