Isolation of polyhydroxyalkanoate-producing bacteria from a polluted soil and characterization of the isolated strain Bacillus cereus YB-4

被引：50

作者：

Mizuno, Kouhei ^{[1
]}

Ohta, Aya ^{[1
]}

Hyakutake, Manami ^{[2
]}

Ichinomiya, Yousuke ^{[2
]}

Tsuge, Takeharu ^{[2
]}

机构：

[1] Kitakyushu Natl Coll Technol, Dept Mat Sci & Chem Engn, Div Biochem Engn, Kokuraminami Ku, Kitakyushu, Fukuoka 8020985, Japan

[2] Tokyo Inst Technol, Dept Innovat & Engn Mat, Midori Ku, Yokohama, Kanagawa 2268502, Japan

来源：

POLYMER DEGRADATION AND STABILITY | 2010年 / 95卷 / 08期

关键词：

Polyhydroxyalkanoate; Bacillus; Molecular weight change; Polluted soil; Isolation; BETA-HYDROXYBUTYRIC ACID; SP INT005; IDENTIFICATION; BIOSYNTHESIS; GENES; 3-HYDROXYVALERATE; SYNTHASE; PHB;

D O I：

10.1016/j.polymdegradstab.2010.01.033

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

We describe the characterization of polyhydroxyalkanoate (PHA)-producing bacteria isolated from an ammunition-polluted soil in Kitakyushu City, Japan. Over 270 strains were evaluated for PHA accumulation based on a colony staining method using Nile red. Of these, nine strains were selected based on the intensity of Nile red fluorescence and the cells were quantitatively analyzed for PHA by gas chromatography. PHA accumulation was observed in five strains, all of which are inferred to be close to the Bacillus cereus group according to 16S rDNA sequence analysis. Interestingly, these strains produced a PHA copolymer, poly(3-hydroxybutyrae-co-3-hydroxyvalerate) [P(3HB-co-3HV)], with a 3HV fraction up to 2 mol% with glucose as a carbon source. Further characterization was performed on one isolate, B. cereus YB-4. Gel permeation chromatography analysis revealed that the number of average molecular weights of PHA accumulated in B. cereus YB-4 drastically changed from 722,000 to 85,000 over a 72-h cultivation period. Furthermore, the PHA synthase genes were cloned and the deduced amino acid sequences were determined. This study provides new insights into PHA biosynthesis by members of the B. cereus group. (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：1335 / 1339

页数：5

共 31 条

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