A trifluoromethylphenyl diazirine-based SecinH3 photoaffinity probe

被引：12
作者：
Albertoni, Barbara [1 ]
Hannam, Jeffrey S. [1 ]
Ackermann, Damian [1 ]
Schmitz, Anton [1 ]
Famulok, Michael [1 ]
机构：
[1] Univ Bonn, LIMES Inst, Chem Biol & Med Chem Unit, D-53121 Bonn, Germany
来源：
CHEMICAL COMMUNICATIONS | 2012年 / 48卷 / 09期
关键词：
FUNCTIONAL-GROUP; CELLS; ARF6; ACTIVATION; CYTOHESINS; PROTEOMICS; SECRETION; MOLECULES; ADHESION; BINDING;
D O I：
10.1039/c2cc16477a
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The synthesis of a trifluoromethylphenyl diazirine photoaffinity probe of the cytohesin inhibitor SecinH3 is described. The probe exhibits improved labelling efficiency over a benzophenone-based probe and thus is more suitable for photoaffinity labelling in complex biological samples.
引用
收藏
页码：1272 / 1274
页数：3
相关论文
共 30 条
[1]   Mapping enzyme active sites in complex proteomes [J].
Adam, GC ;
Burbaum, J ;
Kozarich, JW ;
Patricelli, MP ;
Cravatt, BF .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (05) :1363-1368
[2]   Click Chemistry and Bioorthogonal Reactions: Unprecedented Selectivity in the Labeling of Biological Molecules [J].
Best, Michael D. .
BIOCHEMISTRY, 2009, 48 (28) :6571-6584
[3]   Affinity-Based Labeling of Cytohesins with a Bifunctional SecinH3 Photoaffinity Probe [J].
Bi, Xihe ;
Schmitz, Anton ;
Hayallah, Alaa M. ;
Song, Jin-Na ;
Famulok, Michael .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2008, 47 (49) :9565-9568
[4]   RETRACTED: Cytohesins Are Cytoplasmic ErbB Receptor Activators (Retracted article. See vol. 165, pg. 1293, 2016) [J].
Bill, Anke ;
Schmitz, Anton ;
Albertoni, Barbara ;
Song, Jin-Na ;
Heukamp, Lukas C. ;
Walrafen, David ;
Thorwirth, Franziska ;
Verveer, Peter J. ;
Zimmer, Sebastian ;
Meffert, Lisa ;
Schreiber, Arne ;
Chatterjee, Sampurna ;
Thomas, Roman K. ;
Ullrich, Roland T. ;
Lang, Thorsten ;
Famulok, Michael .
CELL, 2010, 143 (02) :201-211
[5]   Development and application of diazirines in biological and synthetic macromolecular systems [J].
Blencowe, A ;
Hayes, W .
SOFT MATTER, 2005, 1 (03) :178-205
[6]  
BRUNNER J, 1980, J BIOL CHEM, V255, P3313
[7]   NEW PHOTOLABELING AND CROSS-LINKING METHODS [J].
BRUNNER, J .
ANNUAL REVIEW OF BIOCHEMISTRY, 1993, 62 :483-514
[8]   Structural basis and mechanism of autoregulation in 3-phosphoinositide-dependent Grp1 family Arf GTPase exchange factors [J].
DiNitto, Jonathan P. ;
Delprato, Anna ;
Lee, Meng-Tse Gabe ;
Cronin, Thomas C. ;
Huang, Shaohui ;
Guilherme, Adilson ;
Czech, Michael P. ;
Lambright, David G. .
MOLECULAR CELL, 2007, 28 (04) :569-583
[9]   Cytohesin-1 regulates human blood neutrophil adhesion to endothelial cells through β2 integrin activation [J].
El azreq, Mohammed-Amine ;
Bourgoin, Sylvain G. .
MOLECULAR IMMUNOLOGY, 2011, 48 (12-13) :1408-1416
[10]   Cytohesin-1 regulates fMLF-mediated activation and functions of the β2 integrin Mac-1 in human neutrophils [J].
El Azreq, Mohammed-Amine ;
Garceau, Valerie ;
Bourgoin, Sylvain G. .
JOURNAL OF LEUKOCYTE BIOLOGY, 2011, 89 (06) :823-836
123