Quantitative reversed-phase high-performance liquid chromatography of procyanidins in Crataegus leaves and flowers

被引：31

作者：

Rohr, GE

Meier, B

Sticher, O

机构：

[1] Swiss Fed Inst Technol, Dept Pharm, CH-8057 Zurich, Switzerland

[2] Zeller AG, CH-8590 Romanshorn, Switzerland

来源：

JOURNAL OF CHROMATOGRAPHY A | 1999年 / 835卷 / 1-2期

关键词：

Crataegus; procyanidins; epicatechin; polyphenols; flavan-3-ols;

D O I：

10.1016/S0021-9673(99)00043-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A quantitative reversed-phase high-performance liquid chromatographic method using UV diode array detection has been developed for the determination of (-)-epicatechin, procyanidins B2 and B5 in Crataegus leaves and flowers. Validation experiments revealed that if evaluations are based on height counts procyanidins B4 also yields good and procyanidin C1 acceptable recovery rates. This uncommon behavior of procyanidins B4 and C1 is postulated to be due to matrix effects. A sample preparation procedure including a filtration step over C-18 cartridges is proposed for quantitative determinations. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：59 / 65

页数：7

共 34 条

[1] PHENOLIC COMPOSITION AND BROWNING SUSCEPTIBILITY OF VARIOUS APPLE CULTIVARS AT MATURITY [J].