Abnormal Distribution of Gamma-Delta T Lymphocytes and Their Subsets in Type 1 Diabetes

Background. It was assumed that T gamma delta lymphocytes (T gamma delta) are involved in the autoimmune destruction of beta cells, presumably as regulatory cells. Objectives. The aim of this paper was to investigate T gamma delta cells mean percentage (%) in peripheral blood in patients with type 1 diabetes (T1DM) at the time of diagnosis and after twelve months of observation. Material and Methods. A total of 41 patients (21 boys, 51.2%) with new-onset T1DM were included. Exclusion criteria were: Other autoimmune disease, neoplasm, and inflammation. The control group comprised of 14 healthy (7 boys, 50.0%), normostenic children, with normal glucose metabolism and negative history of autoimmune disease and/or diabetes in family. Results. The mean T gamma delta % in patients with new-onset T1DM (8.03 +/- 3.80) and after 12-months of follow-up (6.13 +/- 2.15) was lower than in controls (11.23 +/- 6.79), p = 0.042 and p = 0.016, respectively. A depletion of those cells after one year was observed (p = 0.067). Gender did not affect the level of T gamma delta and subsets. No association between T gamma delta and age neither in T1DM nor controls was observed. Conclusions. Our results support the hypotheses that T gamma delta play a role in T1DM pathogenesis. If so, the decrease of those lymphocytes makes the probands more vulnerable to autoaggression. We report here for the first time the further depletion of T gamma delta after one year of insulin treatment, which may be due to the exacerbation of beta cell destruction in the course of insulitis