An Improved Transplantation Strategy for Mouse Mesenchymal Stem Cells in an Acute Myocardial Infarction Model

被引：18

作者：

Jin, Jianliang ^{[1
]}

Zhao, Yingming ^{[2
]}

Tan, Xiao ^{[2
]}

Guo, Chun ^{[1
]}

Yang, Zhijian ^{[2
]}

Miao, Dengshun ^{[1
]}

机构：

[1] Nanjing Med Univ, Res Ctr Bone & Stem Cells, Dept Anat Histol & Embryol, Nanjing, Jiangsu, Peoples R China

[2] Nanjing Med Univ, Affiliated Hosp 1, Dept Cardiol, Nanjing, Jiangsu, Peoples R China

来源：

PLOS ONE | 2011年 / 6卷 / 06期

关键词：

BONE-MARROW; INDUCED APOPTOSIS; PROGENITOR CELLS; REGENERATION; DIFFERENTIATION; HEART; AKT; ANGIOGENESIS; CONTRIBUTES; ACTIVATION;

D O I：

10.1371/journal.pone.0021005

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

To develop an effective therapeutic strategy for cardiac regeneration using bone marrow mesenchymal stem cells (BM-MSCs), the primary mouse BM-MSCs (1(st) BM-MSCs) and 5(th) passage BM-MSCs from beta-galactosidase transgenic mice were respectively intramyocardially transplanted into the acute myocardial infarction (AMI) model of wild type mice. At the 6(th) week, animals/tissues from the 1(st) BM-MSCs group, the 5(th) passage BM-MSCs group, control group were examined. Our results revealed that, compared to the 5(th) passage BM-MSCs, the 1(st) BM-MSCs had better therapeutic effects in the mouse MI model. The 1 st BM-MSCs maintained greater differentiation potentials towards cardiomocytes or vascular endothelial cells in vitro. This is indicated by higher expressions of cardiomyocyte and vascular endothelial cell mature markers in vitro. Furthermore, we identified that 24 proteins were down-regulated and 3 proteins were up-regulated in the 5(th) BM-MSCs in comparison to the 1(st) BM-MSCs, using mass spectrometry following two-dimensional electrophoresis. Our data suggest that transplantation of the 1(st) BM-MSCs may be an effective therapeutic strategy for cardiac tissue regeneration following AMI, and altered protein expression profiles between the 1(st) BM-MSCs and 5(th) passage BM-MSCs may account for the difference in their maintenance of stemness and their therapeutic effects following AMI.

引用

页数：16

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