Selective efficacy of culture media recommended for isolation and enumeration of Fusarium spp.

被引：21

作者：

Bragulat, MR ^{[1
]}

Martínez, E ^{[1
]}

Castellá, G ^{[1
]}

Cabañes, FJ ^{[1
]}

机构：

[1] Univ Autonoma Barcelona, Fac Vet, Dept Sanitat & Anat Anim, E-08193 Barcelona, Spain

来源：

JOURNAL OF FOOD PROTECTION | 2004年 / 67卷 / 01期

关键词：

D O I：

10.4315/0362-028X-67.1.207

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Selective culture media, such as Nash and Snyder medium (NS), dichloran-chloramphenicol peptone agar (DCPA), modified Czapek-Dox agar (MCz), Czapek Dox iprodione dichloran agar (CZID), potato dextrose iprodione dichloran agar (PDID), or malachite green agar (MGA 2.5), have been developed for isolating and enumerating Fusarium spp. from natural samples. However, some of these culture media are not very selective because they allow the growth of many other fungal species. In this study, a comparison of the selective efficacy of these culture media, using different strains of Fusarium spp. (F. anthophilum, F. culmorum, F. dlamini, F. graminearum, F. napiforme, F. nygamai, F. oxysporum, F. proliferatum, F. semitectum, F. solani, F. subglutinans, and F. verticillioides) and natural samples has been carried out. Among the six recommended selective culture media assayed, no statistical differences were detected in colony counts of the Fusarium spp. strains tested, although the colony diameters in MGA 2.5 were significantly lower than in NS, MCz, DCPA, CZID, and PDID media. With natural samples, MGA 2.5 performs as a potent selective medium for Fusarium spp., whereas the other recommended selective media allow the growth of many other different fungal species including Zygomycetes and yeasts.

引用

页码：207 / 211

页数：5

共 20 条

[1] CZAPEK-DOX AGAR CONTAINING IPRODIONE AND DICLORAN AS A SELECTIVE MEDIUM FOR THE ISOLATION OF FUSARIUM SPECIES [J].

ABILDGREN, MP ;

LUND, F ;

THRANE, U ;

ELMHOLT, S .

LETTERS IN APPLIED MICROBIOLOGY, 1987, 5 (04) :83-86

[2] SELECTIVE MEDIUM FOR ISOLATION OF FUSARIUM SPECIES AND DEMATIACEOUS HYPHOMYCETES FROM CEREALS [J].

ANDREWS, S ;

PITT, JI .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1986, 51 (06) :1235-1238

[3] FUNGAL COLONIZATION OF CORN GROWN IN NEBRASKA IN RELATION TO YEAR, GENOTYPE AND GROWING CONDITIONS [J].

ARINO, AA ;

BULLERMAN, LB .

JOURNAL OF FOOD PROTECTION, 1994, 57 (12) :1084-1087

[4] DYES AS FUNGAL INHIBITORS - EFFECT ON COLONY DIAMETER [J].

BRAGULAT, MR ;

ABARCA, ML ;

BRUGUERA, MT ;

CABANES, FJ .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (09) :2777-2780

[5] DYES AS FUNGAL INHIBITORS - EFFECT ON COLONY ENUMERATION [J].

BRAGULAT, MR ;

ABARCA, ML ;

CASTELLA, G ;

CABANES, FJ .

JOURNAL OF APPLIED BACTERIOLOGY, 1995, 79 (05) :578-582

[6]

BULLERMAN LB, 1992, DEV FOOD SCI, V31, P293

[7] Malachite green agar, a new selective medium for Fusarium spp. [J].

Castella, G ;

Bragulat, MR ;

Rubiales, MV ;

Cabanes, FJ .

MYCOPATHOLOGIA, 1997, 137 (03) :173-178

[8] IMPROVED SELECTIVE MEDIA FOR ISOLATION OF TRICHODERMA SPP OR FUSARIUM SPP [J].

ELAD, Y ;

CHET, I .

PHYTOPARASITICA, 1983, 11 (01) :55-58

[9]

Hall R., 1981, Canadian Journal of Plant Pathology, V3, P47

[10]

KOMADA H, 1975, Review of Plant Protection Research, V8, P114

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