Toward high-throughput chip calorimetry by use of segmented-flow technology

被引：24

作者：

Wolf, A. ^{[1
]}

Hartmann, T. ^{[1
]}

Bertolini, M. ^{[2
]}

Schemberg, J. ^{[3
]}

Grodrian, A. ^{[3
]}

Lemke, K. ^{[3
]}

Foerster, T. ^{[3
]}

Kessler, E. ^{[4
]}

Haenschke, F. ^{[4
]}

Mertens, F. ^{[1
]}

Paus, R. ^{[2
,5
]}

Lerchner, J. ^{[1
]}

机构：

[1] TU Bergakademie Freiberg, Inst Phys Chem, D-09596 Freiberg, Germany

[2] Univ Munster, Dept Dermatol, Munster, Germany

[3] Analyt Measurement Techn eV, Inst Bioproc, Heilbad Heiligenstadt, Germany

[4] IPHT eV, Inst Photon Technol, Jena, Germany

[5] Univ Manchester, Sch Translat Med, Manchester M13 9PL, Lancs, England

来源：

THERMOCHIMICA ACTA | 2015年 / 603卷

关键词：

Chip calorimetry; Segmented-flow technology; Biofouling; Hair follicles; Spheroids; MICROFLUIDICS; GENERATION; GROWTH;

D O I：

10.1016/j.tca.2014.10.021

中图分类号：

O414.1 [热力学];

学科分类号：

摘要：

The adaptation of segmented-flow technology to flow-through calorimetry was demonstrated by different kinds of newly designed chip calorimeters useful for liquids and suspensions as well as for solid samples. In segmented-flow technology, sample material is suspended in aqueous segments of nano- or microliter volume and transported by a water-immiscible carrier liquid. The analysis of the signal dynamics given by segmented samples in flow led to optimal settings of flow rate and sample volume for maximal throughput. For 12 mu L sample segments, a cycle time of 4 min could be achieved. The protection of the measuring chamber of the calorimeters against biofouling caused by the water-immiscible carrier liquid was verified for segmented bacterial samples. The unique possibility to measure solid and aggregated samples in flow-through was demonstrated by the investigation of human hair follicles and fibroblast spheroids. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：172 / 183

页数：12

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