Effects of a high-cultivation temperature on the physiology of three different Yarrowia lipolytica strains

被引：10

作者：

Hackenschmidt, S. ^{[1
]}

Bracharz, F. ^{[1
]}

Daniel, R. ^{[2
]}

Thurmer, A. ^{[3
]}

Bruder, S. ^{[1
]}

Kabisch, J. ^{[1
]}

机构：

[1] Tech Univ Darmstadt, Comp Gestutzte Synthet Biol, Schnittspahnstr 10, D-64287 Darmstadt, Germany

[2] Georg August Univ Gottingen, Dept Genom & Appl Microbiol, Inst Microbiol & Genet, Grisebachstr 8, D-37077 Gottingen, Germany

[3] Robert Koch Inst Berlin, MF 2 Genomsequenzierung, Seestr 10, D-13353 Berlin, Germany

来源：

FEMS YEAST RESEARCH | 2019年 / 19卷 / 07期

关键词：

Yarrowia lipolytica; elevated temperature; cytometry; genome sequencing; polyols; CITRIC-ACID PRODUCTION; WHOLE-GENOME SEQUENCE; LIPID-ACCUMULATION; OXIDATIVE STRESS; RAW GLYCEROL; YEAST; GROWTH; ERYTHRITOL; GENES; OPTIMIZATION;

D O I：

10.1093/femsyr/foz068

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Despite the increasing relevance, ranging from academic research to industrial applications, only a limited number of non-conventional, oleaginous Yarrowia lipolytica strains are characterized in detail. Therefore, we analyzed three strains in regard to their metabolic and physiological properties, especially with respect to important characteristics of a production strain. By investigating different cultivation conditions and media compositions, similarities and differences between the distinct strain backgrounds could be derived. Especially sugar alcohol production, as well as an agglomeration of cells were found to be connected with growth at high temperatures. In addition, sugar alcohol production was independent of high substrate concentrations under these conditions. To investigate the genotypic basis of particular traits, including growth characteristics and metabolite concentrations, genomic analysis were performed. We found sequence variations for one third of the annotated proteins but no obvious link to all phenotypic features.

引用

页数：11

共 85 条

[1] Metabolic engineering in the host Yarrowia lipolytica [J].

Abdel-Mawgoud, Ahmad M. ;

Markham, Kelly A. ;

Palmer, Claire M. ;

Liu, Nian ;

Stephanopoulos, Gregory ;

Alper, Hal S. .

METABOLIC ENGINEERING, 2018, 50 :192-208

[2] Engineering for biofuels: exploiting innate microbial capacity or importing biosynthetic potential? [J].

Alper, Hal ;

Stephanopoulos, Gregory .

NATURE REVIEWS MICROBIOLOGY, 2009, 7 (10) :715-723

[3] Lipids from yeasts and fungi: physiology, production and analytical considerations [J].

Athenaki, M. ;

Gardeli, C. ;

Diamantopoulou, P. ;

Tchakouteu, S. S. ;

Sarris, D. ;

Philippoussis, A. ;

Papanikolaou, S. .

JOURNAL OF APPLIED MICROBIOLOGY, 2018, 124 (02) :336-367

[4]

Barth G.a.G.C., 1996, HANDBOOK, P313, DOI DOI 10.1007/978-3-642-79856-6_10

[5] Microalgal lipids biochemistry and biotechnological perspectives [J].

Bellou, Stamatia ;

Baeshen, Mohammed N. ;

Elazzazy, Ahmed M. ;

Aggeli, Dimitra ;

Sayegh, Fotoon ;

Aggelis, George .

BIOTECHNOLOGY ADVANCES, 2014, 32 (08) :1476-1493

[6]

Beopoulos A., 2010, Handbook of Hydrocarbon and Lipid Microbiology, P2111, DOI [DOI 10.1007/978-3-540-77587-4_152, DOI 10.1007/978-3-540-77587-4152]

[7] Yarrowia lipolytica as a model for bio-oil production [J].

Beopoulos, Athanasios ;

Cescut, Julien ;

Haddouche, Ramdane ;

Uribelarrea, Jean-Louis ;

Molina-Jouve, Carole ;

Nicaud, Jean-Marc .

PROGRESS IN LIPID RESEARCH, 2009, 48 (06) :375-387

[8] Control of Lipid Accumulation in the Yeast Yarrowia lipolytica [J].

Beopoulos, Athanasios ;

Mrozova, Zuzana ;

Thevenieau, France ;

Le Dall, Marie-Therese ;

Hapala, Ivan ;

Papanikolaou, Seraphim ;

Chardot, Thierry ;

Nicaud, Jean-Marc .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2008, 74 (24) :7779-7789

[9]

Biriukova E N, 2008, Mikrobiologiia, V77, P448

[10] Adaptation of the yeast Yarrowia lipolytica to heat shock [J].

Biryukova, E. N. ;

Medentsev, A. G. ;

Arinbasarova, A. Yu. ;

Akimenko, V. K. .

MICROBIOLOGY, 2007, 76 (02) :158-163

← 1 2 3 4 5 6 7 8 9 →