In vitro regeneration from mature leaf explants of Pelargonium rapaceum (L.) L'Herit

Methods to regenerate whole plants from mature leaf explants of Pelargonium rapaceum (I.) L'Heirit were established. To optimize shoot induction, leaf explains were cultured on media containing different types and combinations of plant growth regularors Growth was initiated within 17-24 days culture, and included callus formation. and root or shoot organogenesis ranging from 20 to 100% regeneralion Shoots were induced only when explants were cultivated on MS medium containing a combination of NAA and kinetin, NAA and BAP, IAA and Kinetin. or IAA and BAP On media containing NAA and BAP, dal k incubation was critical for efficient direct shoot regeneration from explants Direct shoot formation and the highest number of shoots per explant (17 6) were obtained from leaf explants cultured in the clar k for 30 days on MS medium containing 0.1 mgl(-1) NAA and 0.1 mgl(-1) BAP. Shoots cultured on MS medium containing 0 1 mgl(-1) NAA formed tuberous roots with microtubers within 42 days Healthy regenerated plants were acclimated and transferred to a greenhouse Crown Copyright (C) 2010 Published by Elsevier B V All rights reserved