Glycoconjugate probes containing a core-fucosylated N-glycan trisaccharide for fucose lectin identification and purification

被引：5

作者：

Cai, Deqin ^{[1
,2
]}

Xun, Chaochao ^{[1
,3
]}

Tang, Feng ^{[1
,2
]}

Tian, Xiaobo ^{[1
]}

Yang, Liyun ^{[1
]}

Ding, Kan ^{[1
]}

Li, Wenzhe ^{[4
]}

Le, Zhiping ^{[3
]}

Huang, Wei ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Shanghai Inst Mat Med, CAS Key Lab Receptor Res, 555 Zuchongzhi Rd, Shanghai 201203, Peoples R China

[2] Univ Chinese Acad Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China

[3] Nanchang Univ, Dept Chem, 999 Xuefu Ave, Nanchang 330031, Jiangxi, Peoples R China

[4] Dalian Med Univ, Coll Basic Med Sci, 9 Western Sect,Lvshun South Rd, Dalian 116044, Liaoning, Peoples R China

来源：

CARBOHYDRATE RESEARCH | 2017年 / 449卷

基金：

中国国家自然科学基金;

关键词：

Core-fucose; Glycoconjugate; Glyco-dendrimer; Glyco-agarose; Fucose-specific lectin; ALPHA-FETOPROTEIN; HEPATOCELLULAR-CARCINOMA; PSEUDOMONAS-AERUGINOSA; HIGH-AFFINITY; GLYCOSYLATION; BINDING; LIGANDS; BLOOD; ACIDS; IGG;

D O I：

10.1016/j.carres.2017.07.011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Glyco-PAMAM dendrimers and glyco-agarose beads bearing a core-fucosylated N-glycan trisaccharide GlcNAc beta 1,4(Fuc alpha 1,6) GlcNAc or a non-fucose disaccharide GlcNAc beta 1,4GlcNAc were successfully synthesized and characterized by monosaccharide analysis with HPAEC-PAD technique. These glycoconjugates as fucose lectin probes were applied in fucose-specific lectin detection and purification. The model fucose lectin AAL indicated binding activity with the FITC-labeled PAMAM carrying core-fucose trisaccharide. An affinity chromatography column stuffed with the agarose beads carrying core-fucosylated trisaccharide exhibited a good specificity in purification of AAL than non-fucose disaccharide agarose beads. These novel glycoconjugates bearing the precise and simplified core-fucose N-glycan structure provided a potential application for core-fucose-specific lectin discovery. (C) 2017 Elsevier Ltd. All rights reserved.

引用

页码：143 / 152

页数：10

共 38 条

[1] Convergent Synthesis of Homogeneous Glc1Man9GlcNAc2-Protein and Derivatives as Ligands of Molecular Chaperones in Protein Quality Control [J].

Amin, Mohammed N. ;

Huang, Wei ;

Mizanur, Rahman M. ;

Wang, Lai-Xi .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2011, 133 (36) :14404-14417

[2] Cell membrane glycosylation mediates the adhesion, migration, and invasion of ovarian carcinoma cells [J].

Casey, RC ;

Oegema, TR ;

Skubitz, KM ;

Pambuccian, SE ;

Grindle, SM ;

Skubitz, APN .

CLINICAL & EXPERIMENTAL METASTASIS, 2003, 20 (02) :143-152

[3] Potential roles of N-glycosylation in cell adhesion [J].

Gu, Jianguo ;

Isaji, Tomoya ;

Xu, Qingsong ;

Kariya, Yoshinobu ;

Gu, Wei ;

Fukuda, Tomohiko ;

Du, Yuguang .

GLYCOCONJUGATE JOURNAL, 2012, 29 (8-9) :599-607

[4]

HARDY MR, 1989, METHOD ENZYMOL, V179, P76

[5] Intracellular functions of N-linked glycans [J].

Helenius, A ;

Aebi, M .

SCIENCE, 2001, 291 (5512) :2364-2369

[6] Chemoenzymatic Glycoengineering of Intact IgG Antibodies for Gain of Functions [J].

Huang, Wei ;

Giddens, John ;

Fan, Shu-Quan ;

Toonstra, Christian ;

Wang, Lai-Xi .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2012, 134 (29) :12308-12318

[7] Unusual Transglycosylation Activity of Flavobacterium meningosepticum Endoglycosidases Enables Convergent Chemoenzymatic Synthesis of Core Fucosylated Complex N-Glycopeptides [J].

Huang, Wei ;

Li, Jie ;

Wang, Lai-Xi .

CHEMBIOCHEM, 2011, 12 (06) :932-941

[8] Fucosyltransferase 1 mediates angiogenesis, cell adhesion and rheumatoid arthritis synovial tissue fibroblast proliferation [J].

Isozaki, Takeo ;

Ruth, Jeffrey H. ;

Amin, Mohammad A. ;

Campbell, Phillip L. ;

Tsou, Pei-Suen ;

Ha, Christine M. ;

Haines, G. Kenneth, III ;

Edhayan, Gautam ;

Koch, Alisa E. .

ARTHRITIS RESEARCH & THERAPY, 2014, 16 (01)

[9] Significant Impact of Single N-Glycan Residues on the Biological Activity of Fc-based Antibody-like Fragments [J].

Jez, Jakub ;

Antes, Bernhard ;

Castilho, Alexandra ;

Kainer, Manuela ;

Wiederkum, Susanne ;

Grass, Josephine ;

Rueker, Florian ;

Woisetschlaeger, Max ;

Steinkellner, Herta .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2012, 287 (29) :24313-24319

[10] PURIFICATION OF THE LEWIS BLOOD-GROUP GENE ASSOCIATED ALPHA-3/4-FUCOSYL-TRANSFERASE FROM HUMAN-MILK - AN ENZYME TRANSFERRING FUCOSE PRIMARILY TO TYPE-1 AND LACTOSE-BASED OLIGOSACCHARIDE CHAINS [J].

JOHNSON, PH ;

WATKINS, WM .

GLYCOCONJUGATE JOURNAL, 1992, 9 (05) :241-249

← 1 2 3 4 →