Overexpression of maize mitogen-activated protein kinase gene, ZmSIMK1 in Arabidopsis increases tolerance to salt stress

被引：70

作者：

Gu, Lingkun ^{[1
]}

Liu, Yukun ^{[1
]}

Zong, Xiaojuan ^{[1
]}

Liu, Lixia ^{[2
]}

Li, Da-Peng ^{[3
]}

Li, De-Quan ^{[1
]}

机构：

[1] Shandong Agr Univ, Coll Life Sci, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China

[2] Dezhou Univ, Dept Agron, Dezhou 253023, Shandong, Peoples R China

[3] Shandong Agr Univ, Coll Food Sci, Tai An 271018, Shandong, Peoples R China

来源：

MOLECULAR BIOLOGY REPORTS | 2010年 / 37卷 / 08期

基金：

中国国家自然科学基金;

关键词：

Gene structure; Maize; Mitogen-activated protein kinase; Salt stress; Transgenic Arabidopsis; MAP KINASE; ABSCISIC-ACID; ANTIOXIDANT DEFENSE; CASCADE; EXPRESSION; COLD;

D O I：

10.1007/s11033-010-0066-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mitogen-activated protein kinase (MAPK) cascades play a remarkably crucial role in plants. It has been studied intensively in model plants Arabidopsis, tobacco and rice. However, the function of MAPKs in maize (Zea mays L.) has not been well documented. ZmSIMK1 (Zea mays salt-induced mitogen-activated protein kinase 1) is a previously identified MAPK gene in maize. In this research, we charactered ZmSIMK1 and showed that ZmSIMK1 was involved in Arabidopsis salt stress. The genomic organization of ZmSIMK1 gene and its expression in maize have been analyzed. In order to investigate the function of ZmSIMK1, we generated transgenic Arabidopsis constitutively overexpressing ZmSIMK1. Ectopic expression of ZmSIMK1 in Arabidopsis resulted in increased resistance against salt stress. Importantly, ZmSIMK1-overexpressing Arabidopsis exhibited constitutive expression of stress-responsive marker genes, RD29A and P5CS1. Furthermore, RD29A and P5CS1 were upregulated under salt stress. These results suggest that ZmSIMK1 may play an important role in plant salt stress.

引用

页码：4067 / 4073

页数：7

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