Overexpression of maize mitogen-activated protein kinase gene, ZmSIMK1 in Arabidopsis increases tolerance to salt stress

被引：70
|
作者：
Gu, Lingkun [1 ]
Liu, Yukun [1 ]
Zong, Xiaojuan [1 ]
Liu, Lixia [2 ]
Li, Da-Peng [3 ]
Li, De-Quan [1 ]
机构：
[1] Shandong Agr Univ, Coll Life Sci, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China
[2] Dezhou Univ, Dept Agron, Dezhou 253023, Shandong, Peoples R China
[3] Shandong Agr Univ, Coll Food Sci, Tai An 271018, Shandong, Peoples R China
来源：
MOLECULAR BIOLOGY REPORTS | 2010年 / 37卷 / 08期
基金：
中国国家自然科学基金;
关键词：
Gene structure; Maize; Mitogen-activated protein kinase; Salt stress; Transgenic Arabidopsis; MAP KINASE; ABSCISIC-ACID; ANTIOXIDANT DEFENSE; CASCADE; EXPRESSION; COLD;
D O I：
10.1007/s11033-010-0066-6
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Mitogen-activated protein kinase (MAPK) cascades play a remarkably crucial role in plants. It has been studied intensively in model plants Arabidopsis, tobacco and rice. However, the function of MAPKs in maize (Zea mays L.) has not been well documented. ZmSIMK1 (Zea mays salt-induced mitogen-activated protein kinase 1) is a previously identified MAPK gene in maize. In this research, we charactered ZmSIMK1 and showed that ZmSIMK1 was involved in Arabidopsis salt stress. The genomic organization of ZmSIMK1 gene and its expression in maize have been analyzed. In order to investigate the function of ZmSIMK1, we generated transgenic Arabidopsis constitutively overexpressing ZmSIMK1. Ectopic expression of ZmSIMK1 in Arabidopsis resulted in increased resistance against salt stress. Importantly, ZmSIMK1-overexpressing Arabidopsis exhibited constitutive expression of stress-responsive marker genes, RD29A and P5CS1. Furthermore, RD29A and P5CS1 were upregulated under salt stress. These results suggest that ZmSIMK1 may play an important role in plant salt stress.
引用
收藏
页码：4067 / 4073
页数：7
相关论文
共 50 条
  • [1] Overexpression of maize mitogen-activated protein kinase gene, ZmSIMK1 in Arabidopsis increases tolerance to salt stress
    Lingkun Gu
    Yukun Liu
    Xiaojuan Zong
    Lixia Liu
    Da-Peng Li
    De-Quan Li
    Molecular Biology Reports, 2010, 37 : 4067 - 4073
  • [2] A maize mitogen-activated protein kinase kinase, ZmMKK1, positively regulated the salt and drought tolerance in transgenic Arabidopsis
    Cai, Guohua
    Wang, Guodong
    Wang, Li
    Liu, Yang
    Pan, Jiaowen
    Li, Dequan
    JOURNAL OF PLANT PHYSIOLOGY, 2014, 171 (12) : 1003 - 1016
  • [3] The overexpression of a maize mitogen-activated protein kinase gene (ZmMPK5) confers salt stress tolerance and induces defence responses in tobacco
    Zhang, D.
    Jiang, S.
    Pan, J.
    Kong, X.
    Zhou, Y.
    Liu, Y.
    Li, D.
    PLANT BIOLOGY, 2014, 16 (03) : 558 - 570
  • [4] Overexpression of tomato mitogen-activated protein kinase SlMPK3 in tobacco increases tolerance to low temperature stress
    Yu, Li
    Yan, Jun
    Yang, Yanjuan
    Zhu, Weimin
    PLANT CELL TISSUE AND ORGAN CULTURE, 2015, 121 (01) : 21 - 34
  • [5] Overexpression of tomato mitogen-activated protein kinase SlMPK3 in tobacco increases tolerance to low temperature stress
    Li Yu
    Jun Yan
    Yanjuan Yang
    Weimin Zhu
    Plant Cell, Tissue and Organ Culture (PCTOC), 2015, 121 : 21 - 34
  • [6] Overexpression of Medicago mitogen-activated protein kinase kinase SIMKK in Arabidopsis causes salt stress-induced subcellular relocation and enhanced salt sensitivity
    Ovecka, Miroslav
    Takac, Tomas
    Vyplelova, Petra
    Komis, Georgios
    Bekesova, Slavka
    Luptovciak, Ivan
    Vadovic, Pavol
    Samajova, Olga
    Hirt, Heribert
    Samaj, Jozef
    NEW BIOTECHNOLOGY, 2016, 33 : S159 - S160
  • [7] Overexpression of Populus trichocarpa Mitogen-Activated Protein Kinase Kinase4 Enhances Salt Tolerance in Tobacco
    Yang, Chengjun
    Wang, Ruoning
    Gou, Luzheng
    Si, Yongchao
    Guan, Qingjie
    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2017, 18 (10)
  • [8] Overexpression of constitutively active mitogen activated protein kinase kinase 6 enhances tolerance to salt stress in rice
    Kumar, Kundan
    Sinha, Alok Krishna
    RICE, 2013, 6 : 1 - 5
  • [9] Overexpression of constitutively active mitogen activated protein kinase kinase 6 enhances tolerance to salt stress in rice
    Kundan Kumar
    Alok Krishna Sinha
    Rice, 2013, 6
  • [10] Enhanced Tolerance to Chilling Stress in Tomato by Overexpression of a Mitogen-Activated Protein Kinase, SlMPK7
    Li Yu
    Jun Yan
    Yanjuan Yang
    Lizhong He
    Weimin Zhu
    Plant Molecular Biology Reporter, 2016, 34 : 76 - 88
12345