Effects of sertoli cell-conditioned medium on ventral midbrain neural stem cells: A preliminary report

被引：14
作者：
Shamekh, Rania [1 ]
Saporta, Samuel [2 ,3 ]
Cameron, Don F. [2 ]
Willing, Alison E. [1 ,2 ,3 ]
Sanberg, Cindy D. [4 ]
Johe, Karl [5 ]
Sanberg, P. R. [1 ,3 ]
机构：
[1] Univ S Florida, Coll Med, Ctr Excellence Aging & Brain Repair, Dept Neurosurg, Tampa, FL 33612 USA
[2] Univ S Florida, Coll Med, Ctr Excellence Aging & Brain Repair, Dept Pathol & Cell Biol, Tampa, FL 33612 USA
[3] Univ S Florida, Coll Med, Ctr Excellence Aging & Brain Repair, Dept Mol Pharmacol & Physiol, Tampa, FL 33612 USA
[4] USF Ctr Entrepreneurship, Saneron CCEL Therapeut, Tampa, FL 33612 USA
[5] NEURALSTEM Inc, Rockville, MD 20850 USA
来源：
NEUROTOXICITY RESEARCH | 2008年 / 13卷 / 3-4期
关键词：
sertoli cells; stem cells; Parkinson's disease; fetal tissue; growth factors;
D O I：
10.1007/BF03033507
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
The 796RMB cell line is a multipotent stem cell line isolated from human fetal midbrain tissues, a region from which dopamine neurons of the substantia nigra develop. It would be useful to increase the dopaminergic characteristics of this cell line to enhance its usefulness as a cell therapy for Parkinson's disease utilizing transplantation protocols. Sertoli cells and its conditioned media isolated from the testis have been previously shown to enhance tyrosine hydroxylase expression in ventral mesencephalon neurons both in vitro and in vivo. Therefore, the present preliminary study investigated the ability of Sertoli cell pre-conditioned medium to enhance differentiation of the 796MB cell line toward the domaminergic phenotype. Results showed that secretory products derived from Sertoli cell conditioned medium increased cell proliferation and enhanced dopaminergic neuronal differentiation of the 796RMB cell line. These findings may lead to alternative therapeutic cell transplantation protocols for the treatment of Parkinson's disease.
引用
收藏
页码：241 / 246
页数：6
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