In vivo patch-clamp recording from locus coeruleus neurones in the rat brainstem

被引：31

作者：

Sugiyama, Daisuke ^{[1
,2
]}

Hur, Sung Won ^{[1
,3
,4
]}

Pickering, Anthony E. ^{[5
]}

Kase, Daisuke ^{[1
]}

Kim, Sang Jeong ^{[3
,4
]}

Kawamata, Mikito ^{[2
]}

Imoto, Keiji ^{[1
,6
]}

Furue, Hidemasa ^{[1
,6
]}

机构：

[1] Natl Inst Physiol Sci, Dept Informat Physiol, Okazaki, Aichi 4448787, Japan

[2] Shinshu Univ, Sch Med, Dept Anesthesiol & Resuscitol, Matsumoto, Nagano 3908621, Japan

[3] Seoul Natl Univ, Coll Med, Dept Physiol, Seoul 110799, South Korea

[4] Seoul Natl Univ, Coll Med, Dept Brain & Cognit Sci, Seoul 110799, South Korea

[5] Univ Bristol, Sch Physiol & Pharmacol, Bristol BS8 1TD, Avon, England

[6] Grad Univ Adv Studies SOKENDAI, Sch Life Sci, Okazaki, Aichi 4448787, Japan

来源：

JOURNAL OF PHYSIOLOGY-LONDON | 2012年 / 590卷 / 10期

基金：

新加坡国家研究基金会;

关键词：

NORADRENERGIC NEURONS; MEMBRANE;

D O I：

10.1113/jphysiol.2011.226407

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Locus coeruleus (LC) neurones extend noradrenergic projections throughout the neuroaxis and are involved in homeostatic functions such as pain modulation, arousal and cardio-respiratory control. To address the cellular mechanisms underlying pain modulation we have developed a patch-clamp recording technique from LC neurones in anaesthetized rats. These recordings showed LC discharge in vivo to be driven by both spontaneous membrane potential oscillations and CNQX-sensitive EPSCs opposed by bicuculine-sensitive IPSCs. Hindlimb pinch evoked a biphasic action potential response underpinned by a slow monophasic excitatory current. This approach allows detailed characterisation of the synaptic and integrative mechanisms of LC responses to naturalistic stimulation.

引用

页码：2225 / 2231

页数：7

共 12 条

[1]

Aston-Jones G., 2004, RAT NERVOUS SYSTEM, P259, DOI DOI 10.1016/B978-012547638-6/50012-2

[2] ENDOGENOUS PAIN CONTROL MECHANISMS - REVIEW AND HYPOTHESIS [J].