A study on the role that quorum sensing play in antibiotic-resistant plasmid conjugative transfer in Escherichia coli

被引：28

作者：

Zhang, Yueheng ^{[1
,2
]}

Ma, Qingping ^{[1
,2
]}

Su, Bingmei ^{[3
]}

Chen, Rui ^{[1
]}

Lin, Juan ^{[3
]}

Lin, Zhifen ^{[1
,4
,5
]}

Wang, Dali ^{[1
]}

Yu, Yang ^{[6
]}

机构：

[1] Tongji Univ, Coll Environm Sci & Engn, State Key Lab Pollut Control & Resource Reuse, Shanghai 200092, Peoples R China

[2] Shanghai Ocean Univ, Coll Marine Ecol & Environm, Shanghai 201306, Peoples R China

[3] Fuzhou Univ, Fujian Prov Key Lab Marine Enzyme Engn, Fuzhou 350116, Fujian, Peoples R China

[4] Shanghai Inst Pollut Control & Ecol Secur, Shanghai 200092, Peoples R China

[5] Shanghai Key Lab Chem Assessment & Sustainabil, Shanghai 201306, Peoples R China

[6] Minist Environm Protect, Solid Waste & Chem Management Ctr, Beijing 100029, Peoples R China

来源：

ECOTOXICOLOGY | 2018年 / 27卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Antibiotic resistance genes; Quorum sensing; Plasmid RP4; Conjugative transfer; Escherichia coli; SUBINHIBITORY CONCENTRATIONS; HORIZONTAL TRANSFER; GENE-EXPRESSION; INHIBITION; BACTERIA; CHINA; SOILS; RIVER;

D O I：

10.1007/s10646-017-1886-0

中图分类号：

Q14 [生态学（生物生态学）];

学科分类号：

071012 ; 0713 ;

摘要：

Horizontal genes transfer (HGT) plays an important role in the dissemination of antibiotic resistance genes (ARGs) in the environment. However, the mechanisms of HGT of ARGs under the influence of antibiotics in sub-MIC remain rarely explored. Moreover, given its collective nature, HGT was considered to be relative to quorum sensing (QS) system. To investigate whether QS has any impact on horizontal gene transfer of ARGs, experiments were conducted to determine the conjugative efficiency of plasmid RP4 on Escherichia coli (E.coli) under the influences of tetracyclines (TCs), quorum sensing autoinducers (AIs) and quorum sensing inhibitors (QSIs). The results indicated that the sub-MIC TCs could facilitate the conjugative transfer of RP4, a process which could be enhanced by AIs but inhibited by QSIs. This study demonstrated the roles that QS played in the dissemination of ARGs, and provided theoretical insights into the mechanism of HGT of ARGs in the environment.

引用

页码：209 / 216

页数：8

共 27 条

[1] Autoinducer 2 controls biofilm formation in Escherichia coli through a novel motility quorum-sensing regulator (MqsR, B3022) [J].