Immunolocalization for glucocorticoid receptor and effect of cortisol on in vitro development of preantral follicles

被引：12

作者：

Pontes, Julian T. ^{[1
]}

Maside, Caroline ^{[1
]}

Lima, Laritza F. ^{[1
]}

Magalhaes-Padilha, Deborah M. ^{[2
]}

Padilha, Rodrigo T. ^{[2
]}

Matos, Maria Helena T. ^{[3
]}

Figueiredo, Jose R. ^{[1
]}

Campello, Claudio C. ^{[1
]}

机构：

[1] Univ Estadual Ceara, Lab Manipulat Oocytes Enclosed Preantral Follicle, Av Paranjana 1700,Campus Itaperi, BR-60740000 Fortaleza, CE, Brazil

[2] Univ Potiguar, Laureate Int Univ, Postgrad Biotechnol, Av Senador Salgado Filho 1610, BR-59056000 Natal, CE, Brazil

[3] Fed Univ San Francisco Valley, Nucleus Biotechnol Appl Ovarian Follicle Dev, Petrolina, PE, Brazil

来源：

VETERINARY AND ANIMAL SCIENCE | 2019年 / 7卷

关键词：

Caprine; Cortisol; NR3C1; Preantral follicles; Stress; GENE-EXPRESSION; STRESS; STIMULATION; CULTURE; GROWTH;

D O I：

10.1016/j.vas.2019.100060

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The aim of this study was to evaluate the immunolocalization for glucocorticoid receptor (NR3C1) in goat ovarian follicles and the effect of cortisol on in vitro development of preantral follicles. Goat ovarian fragments were cultured for 7 days under different cortisol concentrations (0, 1, 5 and 10 ng/ml). Before and after culture, the protein expression of NR3C1 was analyzed in ovarian tissue by immunohistochemical analysis. Moreover, the endpoints follicular morphology, viability, activation as well as follicular and oocyte diameter were also analyzed. The NR3C1 was strongly expressed in oocytes of primordial and antral follicles. A progressive increase of immunostaining for NR3C1 in granulosa cells from primordial to antral follicles was observed regardless of the treatment. After in vitro culture, it was observed a significant reduction in the rate of normal preantral follicles rate in the 10 ng/ml cortisol treatment when compared to the other treatments. Moreover, follicular and oocyte diameter significantly decreased in all treatments (cortisol 0, 1, 5 and 10 ng/ml) compared to the fresh control. After culture, the activation rate significantly increased when the follicles were exposed to 1, 5 and 10 ng/ml cortisol compared to the fresh control. In conclusion, it was observed the presence of NR3C1 in the oocyte and granulosa cells in all follicular categories, except in granulosa cells of primordial follicles. The in vitro culture showed that high cortisol concentration (10 ng/ml) exerts a deleterious effect on follicular survival.

引用

页数：5

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