Digital PCR hits its stride

被引：400

作者：

Baker, Monya

机构：

[1] Monya Baker is technology editor for Nature and Nature Methods,

来源：

NATURE METHODS | 2012年 / 9卷 / 06期

关键词：

D O I：

10.1038/nmeth.2027

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

As the less familiar cousin of quantitative PCR moves mainstream, researchers have more options to choose from.

引用

页码：541 / 544

页数：4

共 8 条

[1] Personal Omics Profiling Reveals Dynamic Molecular and Medical Phenotypes [J].

Chen, Rui ;

Mias, George I. ;

Li-Pook-Than, Jennifer ;

Jiang, Lihua ;

Lam, Hugo Y. K. ;

Chen, Rong ;

Miriami, Elana ;

Karczewski, Konrad J. ;

Hariharan, Manoj ;

Dewey, Frederick E. ;

Cheng, Yong ;

Clark, Michael J. ;

Im, Hogune ;

Habegger, Lukas ;

Balasubramanian, Suganthi ;

O'Huallachain, Maeve ;

Dudley, Joel T. ;

Hillenmeyer, Sara ;

Haraksingh, Rajini ;

Sharon, Donald ;

Euskirchen, Ghia ;

Lacroute, Phil ;

Bettinger, Keith ;

Boyle, Alan P. ;

Kasowski, Maya ;

Grubert, Fabian ;

Seki, Scott ;

Garcia, Marco ;

Whirl-Carrillo, Michelle ;

Gallardo, Mercedes ;

Blasco, Maria A. ;

Greenberg, Peter L. ;

Snyder, Phyllis ;

Klein, Teri E. ;

Altman, Russ B. ;

Butte, Atul J. ;

Ashley, Euan A. ;

Gerstein, Mark ;

Nadeau, Kari C. ;

Tang, Hua ;

Snyder, Michael .

CELL, 2012, 148 (06) :1293-1307

[2] Digital PCR for the molecular detection of fetal chromosomal aneuploidy [J].

Lo, Y. M. Dennis ;

Lun, Fiona M. F. ;

Chan, K. C. Allen ;

Tsui, Nancy B. Y. ;

Chong, Ka C. ;

Lau, Tze K. ;

Leung, Tak Y. ;

Zee, Benny C. Y. ;

Cantor, Charles R. ;

Chiu, Rossa W. K. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (32) :13116-13121

[3] Quantitative and sensitive detection of rare mutations using droplet-based microfluidics [J].

Pekin, Deniz ;

Skhiri, Yousr ;

Baret, Jean-Christophe ;

Le Corre, Delphine ;

Mazutis, Linas ;

Ben Salem, Chaouki ;

Millot, Florian ;

El Harrak, Abdeslam ;

Hutchison, J. Brian ;

Larson, Jonathan W. ;

Link, Darren R. ;

Laurent-Puig, Pierre ;

Griffiths, Andrew D. ;

Taly, Valerie .

LAB ON A CHIP, 2011, 11 (13) :2156-2166

[4]

SYKES PJ, 1992, BIOTECHNIQUES, V13, P444

[5] Probing Individual Environmental Bacteria for Viruses by Using Microfluidic Digital PCR [J].

Tadmor, Arbel D. ;

Ottesen, Elizabeth A. ;

Leadbetter, Jared R. ;

Phillips, Rob .

SCIENCE, 2011, 333 (6038) :58-62

[6] Digital PCR [J].

Vogelstein, B ;

Kinzler, KW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (16) :9236-9241

[7] Quantifying EGFR Alterations in the Lung Cancer Genome with Nanofluidic Digital PCR Arrays [J].

Wang, Jun ;

Ramakrishnan, Ramesh ;

Tang, Zhe ;

Fan, Weiwen ;

Kluge, Amy ;

Dowlati, Afshin ;

Jones, Robert C. ;

Ma, Patrick C. .

CLINICAL CHEMISTRY, 2010, 56 (04) :623-632

[8]

Whale A. S., 2012, NUCLEIC ACIDS RES, DOI [10.1093/nar/gks203, DOI 10.1093/NAR/GKS203]

← 1 →