Improved laboratory enrichment for enterohemorrhagic Escherichia coli by exposure to extremely acidic conditions

被引：15

作者：

Grant, MA ^{[1
]}

机构：

[1] US FDA, Bothell, WA 98021 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2004年 / 70卷 / 02期

关键词：

D O I：

10.1128/AEM.70.2.1226-1230.2004

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Analysis of food samples for E. coli O157:H7 using the standard U.S. Food and Drug Administration procedure is frequently complicated by overgrowth of nontarget microorganisms. A new procedure was developed for enrichment of enterohemorrhagic E. coli (EHEC) which utilizes exposure to pH 2.00 for 2 h. This procedure yielded larger populations of EHEC than the standard method by factors ranging from 2.7 to 7.7 and, when age-stressed cultures were used, by factors ranging from 2.7 to 11.5. Cultures of competing enterics were more effectively inhibited by the new enrichment protocol as well.

引用

页码：1226 / 1230

页数：5