Establishment and characterization of human gingival squamous cell carcinoma cell line NOCS-1

被引：1

作者：

Takahashi, Haruka ^{[1
]}

Watanabe, Miho ^{[1
]}

Ohyama, Akihiro ^{[2
,3
]}

Toyomura, Junko ^{[2
,3
]}

Tachibana, Toshiaki ^{[4
]}

Tanaka, Akira ^{[1
]}

Ishikawa, Hiroshi ^{[2
,3
]}

机构：

[1] Nippon Dent Univ, Sch Life Dent Niigata, Dept Oral & Maxillofacial Surg, Chuo Ku, 1-8 Hamaura Cho, Niigata 9518580, Japan

[2] Nippon Dent Univ Tokyo, Sch Life Dent Tokyo, Dept NDU Life Sci, Chiyoda Ku, 1-9-20 Fujimi, Tokyo 1028159, Japan

[3] Univ Tsukuba, Fac Med, Dept Neurosurg, Lab Clin Regenerat Med, 1-1-1 Tennodai, Tsukuba, Ibaraki 3058575, Japan

[4] Jikei Univ, Sch Med, Core Res Facil, Minato Ku, 3-19-18 Nishishinbashi, Tokyo 1058471, Japan

来源：

HUMAN CELL | 2019年 / 32卷 / 01期

关键词：

Cell line; Gingival squamous cell carcinoma; G establishment; Xenograft; Anticancer drug susceptibility tests; LYMPH-NODE; TRANSPLANTATION; TUMOR; GENE; P53;

D O I：

10.1007/s13577-018-0216-z

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The NOCS-1 cell line was established from the left gingiva tumor in an 86-year-old Japanese man. Histopathological diagnosis of the original tumor was well-differentiated squamous cell carcinoma. NOCS-1 cells were adhesive epithelial cells with neoplastic or pleomorphic features and grew without contact inhibition. It has been subcultured 70 times during the past 26months. From passage 3, melanin-containing cells began to be observed in the NOCS-1 cell line. The plating efficiencies were 25% and 23%, doubling times were 29 and 26h, and saturation densities were 6.9x10(4)/cm(2) and 8.7x10(4)/cm(2), at passage 12 and 30, respectively. When NOCS-1 cells were xenotransplanted subcutaneously into SCID mice, they produced tumors that histopathologically resembled the original tumor. In addition, NOCS-1-XG cells derived from the xenotransplanted tumor were similar to NOCS-1 cells. We believe that this cell line may be a valuable tool to develop immunotherapy and chemotherapy regimens.

引用

页码：75 / 82

页数：8