Application of multiple sublethal stress indicators to assess the health of fish in Pamlico Sound following extensive flooding

Multiple indicators of sublethal stress (bioindicators) were used to assess the health and condition of two important estuarine fish species in the Pamlico Sound estuary following extensive flooding from three sequential hurricanes that occurred during early fall 1999. Bioindicators ranging from the biochemical to the reproductive and organism-level were used to assess the health of southern flounder and spot in Pamlico Sound compared to the health of these same species sampled from a relatively unaffected reference site in lower Core Sound. Many of the physiological, reproductive, immunological, histopathological, and general condition indices suggested that both species, and particularly spot, in Pamlico Sound were sublethally stressed and in poorer condition than fish sampled from Core Sound. The major environmental stressors causing these sublethal stress responses in Pamlico Sound fish appears to be those related to episodic hypoxic exposure or a combination of effects associated with hypoxic conditions such as alterations in preferred habitat and food availability. Although fish populations in Pamlico Sound do not appear to be severely damaged or impaired at this time, organisms that are sublethally stressed can incur increased vulnerability to additional or future stressors such as modified physicochemical regimes, changes in food and habitat availability, and increases in infectious pathogens. Because of the low flushing rate (similar to1 yr) of Pamlico Sound, recovery rate may be exceptionally slow, prolonging any adverse effects of altered nutrient regimes (such as hypoxia) on the health and fitness of resident fish populations. Flooding from the 1999 hurricanes may have contributed to the short-term health and condition of finfish species in Pamlico Sound and also influenced longer-term recovery and ecological status of this system. Longer-term manifestation of effects from flooding may be of particular concern as the frequency of hurricanes is expected to increase over the next few years and the accelerated uses of the coastal zone places further stress on estuarine resources.