Wavelet analysis reveals heterogeneous time-dependent oscillations of individual mitochondria

被引：24
作者：
Kurz, Felix T. [1 ,2 ]
Aon, Miguel A. [3 ]
O'Rourke, Brian [3 ]
Armoundas, Antonis A. [1 ]
机构：
[1] Harvard Univ, Sch Med, Cardiovasc Res Ctr, Massachusetts Gen Hosp, Charlestown, MA 02129 USA
[2] Charite, Klin mS Kardiol, D-13353 Berlin, Germany
[3] Johns Hopkins Univ, Inst Mol Cardiobiol, Dept Med, Baltimore, MD USA
来源：
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY | 2010年 / 299卷 / 05期
基金：
美国国家卫生研究院;
关键词：
mitochondrial cluster; frequency; amplitude; mitochondrial oscillator; COUPLED OSCILLATORS; HEART-CELLS; ORGANIZATION; POPULATIONS; CRITICALITY; DYNAMICS; NETWORK; RHYTHMS;
D O I：
10.1152/ajpheart.00640.2010
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Kurz FT, Aon MA, O'Rourke B, Armoundas AA. Wavelet analysis reveals heterogeneous time-dependent oscillations of individual mitochondria. Am J Physiol Heart Circ Physiol 299: H1736-H1740, 2010. First published September 10, 2010; doi:10.1152/ajpheart.00640.2010.-Mitochondrial inner membrane potential oscillations in cardiac myocytes synchronize under oxidative or metabolic stress, leading to synchronized whole cell oscillations. Gaining information about the temporal properties of individual mitochondrial oscillators is essential to comprehend the network's intrinsic spatiotemporal organization. We have developed methods to detect individual mitochondrial tetramethylrhodamine ethyl ester fluorescence oscillations and assess their dynamical properties using wavelet analysis. We demonstrate that these advanced signal processing tools can provide quantitative spatiotemporal information about intermitochondrial coupling. We have found that the mean frequency of selected groups of continuously oscillating mitochondria was 16.49 +/- 1.04 mHz, whereas the mean frequency in the normalized mean global wavelet spectrum was 22.84 +/- 1.80 mHz (n = 9 myocytes). In conclusion, this novel methodology will help shed new light on the dynamical properties of the mitochondrial network on the verge of synchronization.
引用
收藏
页码：H1736 / H1740
页数：5
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