Analysis of Phospholipids, Lysophospholipids, and Their Linked Fatty Acyl Chains in Yellow Lupin Seeds (Lupinus luteus L.) by Liquid Chromatography and Tandem Mass Spectrometry

被引:36
作者
Calvano, Cosima Damiana [1 ,2 ]
Bianco, Mariachiara [3 ]
Ventura, Giovanni [3 ]
Losito, Ilario [1 ,3 ]
Palmisano, Francesco [1 ,3 ]
Cataldi, Tommaso R., I [1 ,3 ]
机构
[1] Univ Bari Aldo Moro, Ctr Interdipartimentale SMART, Via Orabona 4, I-70126 Bari, Italy
[2] Univ Bari Aldo Moro, Dipartimento Farm Sci Farmaco, Via Orabona 4, I-70126 Bari, Italy
[3] Univ Bari Aldo Moro, Dipartimento Chim, Via Orabona 4, I-70126 Bari, Italy
关键词
LC-ESI-tandem MS; food; phospholipids; fatty acids; Lupinus luteus; DRIVEN FRAGMENTATION PROCESSES; ENERGY COLLISIONAL ACTIVATION; THERMALLY STRESSED MUSSELS; ELECTROSPRAY-IONIZATION; PHOSPHATIDIC-ACID; LIPIDS; PHOSPHATIDYLGLYCEROL; IDENTIFICATION; EXTRACTION; SEPARATION;
D O I
10.3390/molecules25040805
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Hydrophilic interaction liquid chromatography (HILIC) and electrospray ionization (ESI) coupled to either Fourier-transform (FT) orbital-trap or linear ion-trap tandem mass spectrometry (LIT-MS/MS) was used to characterize the phospholipidome of yellow lupin (Lupinus luteus) seeds. Phosphatidylcholines (PC) were the most abundant species (41 6%), which were followed by lyso-forms LPC (30 +/- 11%), phosphatidylethanolamines (PE, 13 +/- 4%), phosphatidylglycerols (PG, 5.1 +/- 1.7%), phosphatidic acids (PA, 4.9 +/- 1.8%), phosphatidylinositols (PI, 4.7 +/- 1.1%), and LPE (1.2 0.5%). The occurrence of both isomeric forms of several LPC and LPE was inferred by a well-defined fragmentation pattern observed in negative ion mode. An unprecedented characterization of more than 200 polar lipids including 52 PC, 42 PE, 42 PA, 35 PG, 16 LPC, 13 LPE, and 10 PI, is reported. The most abundant fatty acids (FA) as esterified acyl chains in PL were 18:1 (oleic), 18:2 (linoleic), 16:0 (palmitic), and 18:3 (linolenic) with relatively high contents of long fatty acyl chains such as 22:0 (behenic), 24:0 (lignoceric), 20:1 (gondoic), and 22:1 (erucic). Their occurrence was confirmed by reversed-phase (RP) LC-ESI-FTMS analysis of a chemically hydrolyzed sample extract in acid conditions at 100 degrees C for 45 min.
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页数:20
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