Proteomic analysis of protein-protein interactions using chromosome deletion lines

Genome manipulation has been carried out either by deleting chromosomes - an arm (ditelocentric lines), a part of chromosome (fine deletion), one of the 3 genomes, A, B, D - or by introducing a gene or pair of chromosomes (disomic addition line) in the genome composition of common wheat. The protein network was determined by quantitative analysis of the expressed proteins. Deletion of chromosome(s), either an arm, a part of an arm or one of the 3 genomes, resulted a serious imbalance in the network of protein expression. Whereas, the addition of extra chromosomes, and hence proteins, from the alien species had little effect on the network of protein expression.