Imaging Flies by Fluorescence Microscopy: Principles, Technologies, and Applications

被引：43

作者：

Dunst, Sebastian ^{[1
]}

Tomancak, Pavel ^{[2
]}

机构：

[1] German Fed Inst Risk Assessment, German Ctr Protect Lab Anim Bf3R, D-10589 Berlin, Germany

[2] Max Planck Inst Mol Cell Biol & Genet, Pfotenhauerstr 108, D-01307 Dresden, Germany

来源：

GENETICS | 2019年 / 211卷 / 01期

关键词：

imaging; labeling; transgenic reporters; FlyBook; PROTEIN-PROTEIN INTERACTIONS; MESSENGER-RNA LOCALIZATION; LIGHT-SHEET MICROSCOPY; CELL-SHAPE CHANGES; DROSOPHILA EMBRYOS; HOMOLOGOUS RECOMBINATION; PHOTOACTIVATABLE GFP; EXPRESSION PATTERNS; RESOLUTION LIMIT; MOSAIC ANALYSIS;

D O I：

10.1534/genetics.118.300227

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The development of fluorescent labels and powerful imaging technologies in the last two decades has revolutionized the field of fluorescence microscopy, which is now widely used in diverse scientific fields from biology to biomedical and materials science. Fluorescence microscopy has also become a standard technique in research laboratories working on Drosophila melanogaster as a model organism. Here, we review the principles of fluorescence microscopy technologies from wide-field to Super-resolution microscopy and its application in the Drosophila research field.

引用

页码：15 / 34

页数：20

共 158 条

[1]

Abbe E., 1873, Arch. Mikrosk. Anat, V9, P413, DOI DOI 10.1007/BF02956173

[2] Cell Flow Reorients the Axis of Planar Polarity in the Wing Epithelium of Drosophila [J].

Aigouy, Benoit ;

Farhadifar, Reza ;

Staple, Douglas B. ;

Sagner, Andreas ;

Roeper, Jens-Christian ;

Juelicher, Frank ;

Eaton, Suzanne .

CELL, 2010, 142 (05) :773-786

[3]

Airy G B., 1835, Transactions of the Cambridge Philosophical Society, V5, P283

[4] Enhancer trapping with a red fluorescent protein reporter in Drosophila [J].

Akimoto, A ;

Wada, H ;

Hayashi, S .

DEVELOPMENTAL DYNAMICS, 2005, 233 (03) :993-997

[5] Optogenetic pacing in Drosophila melanogaster [J].

Alex, Aneesh ;

Li, Airong ;

Tanzi, Rudolph E. ;

Zhou, Chao .

SCIENCE ADVANCES, 2015, 1 (09)

[6]

Balla Tamas, 2009, Curr Protoc Cell Biol, VChapter 24, DOI 10.1002/0471143030.cb2404s42

[7] Atypical membrane topology and heteromeric function of Drosophila odorant receptors in vivo [J].

Benton, R ;

Sachse, S ;

Michnick, SW ;

Vosshall, LB .

PLOS BIOLOGY, 2006, 4 (02) :240-257

[8] Localization of ASH1 mRNA particles in living yeast [J].

Bertrand, E ;

Chartrand, P ;

Schaefer, M ;

Shenoy, SM ;

Singer, RH ;

Long, RM .

MOLECULAR CELL, 1998, 2 (04) :437-445

[9] Imaging intracellular fluorescent proteins at nanometer resolution [J].

Betzig, Eric ;

Patterson, George H. ;

Sougrat, Rachid ;

Lindwasser, O. Wolf ;

Olenych, Scott ;

Bonifacino, Juan S. ;

Davidson, Michael W. ;

Lippincott-Schwartz, Jennifer ;

Hess, Harald F. .

SCIENCE, 2006, 313 (5793) :1642-1645

[10] Spatio-Temporal Plasticity in Chromatin Organization in Mouse Cell Differentiation and during Drosophila Embryogenesis [J].

Bhattacharya, Dipanjan ;

Talwar, Shefali ;

Mazumder, Aprotim ;

Shivashankar, G. V. .

BIOPHYSICAL JOURNAL, 2009, 96 (09) :3832-3839

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