A rapid PCR-based assay for identification of cryptic Myotis spp. (M. mystacinus, M. brandtii and M. alcathoe)

被引：10

作者：

Boston, Emma S. M. ^{[1
]}

Hanrahan, Nicola ^{[1
]}

Puechmaille, Sebastien J. ^{[1
]}

Ruedi, Manuel ^{[3
]}

Buckley, Daniel J. ^{[1
]}

Lundy, Mathieu G. ^{[2
]}

Scott, David D. ^{[2
]}

Prodoehl, Paulo A. ^{[2
]}

Montgomery, W. I. ^{[2
]}

Teeling, Emma C. ^{[1
]}

机构：

[1] Univ Coll Dublin, Sch Biol & Environm Sci, Ctr Irish Bat Res, Dublin 4, Ireland

[2] Queens Univ Belfast, Ctr Irish Bat Res, Sch Biol Sci, Belfast BT9 7BL, Antrim, North Ireland

[3] Nat Hist Museum, Geneva, Switzerland

来源：

CONSERVATION GENETICS RESOURCES | 2011年 / 3卷 / 03期

关键词：

Cryptic species; Chiroptera; Myotis; SPECIES IDENTIFICATION; BAT; DNA;

D O I：

10.1007/s12686-011-9404-9

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

The development of a quick PCR-based method to distinguish European cryptic Myotis spp., Myotis mystacinus, Myotis brandtii and Myotis alcathoe is described. Primers were designed around species-specific single nucleotide polymorphisms (SNP's) in the ND1 mitochondrial gene, and a pair of control primers was designed in the 12S mitochondrial gene. A multiplex of seven primer combinations produces clear species-specific bands using gel electrophoresis. Robustness of the method was tested on 33 M. mystacinus, 16 M. brandtii and 15 M. alcathoe samples from across the European range of these species. The method worked well on faecal samples collected from maternity roosts of M. mystacinus. The test is intended to aid collection of data on these species through a rapid and easy identification method with the ability to use DNA obtained from a range of sources including faecal matter.

引用

页码：557 / 563

页数：7

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