Preferential induction of mammary tumors in p53 hemizygous BALB/c mice by fractionated irradiation of a sub-lethal dose of X-rays

被引:12
作者
Mori, N
Yamate, J
Umesako, SI
Hong, DP
Okumoto, M
Nakao, R
机构
[1] Univ Osaka Prefecture, Res Inst Adv Sci & Technol, Sakai, Osaka 5998570, Japan
[2] Univ Osaka Prefecture, Dept Vet Pathol, Grad Sch Agr Lifesci, Sakai, Osaka 5998531, Japan
关键词
mammary tumors; BALB; c mice; p53; deficiency; radiation; tumor susceptibility;
D O I
10.1269/jrr.44.249
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
BALB/c mice are susceptible to radiation-induced mammary tumors as well as lymphomas. We investigated the effects of the p53 deficient allele and of X-irradiation on the tumor spectrum in the BALB/c background. Substantially all p53 -/- animals died of thymic lymphomas before 36 weeks of age, while none of the p53 +/+ animals died during that period. At this age, mortalities of p53 +/- females and males were 5% (1/22) and 11% (1/9), respectively, due to non-thymic lymphoma and sarcoma. When exposed to 4 Gy of X-irradiation, 100% (44/44) and 95% (18/19) of p53 +/- mice died with tumors within 36 weeks. Among these, the predominant cause of death was lymphoma in either sex [26/44 (59%) in females; 13/19 (68%) in males]; mammary adenocarcinoma (15/44, 34%) and sarcoma (3/19, 16%) were semi-dominant in females and males, respectively. The mortalities of similarly treated p53 +/+ mice were 16% (5/31) in females and 17% (3/18) in males: virtually all deaths were due to thymic lymphomas in either sex. When exposed to 4 x 0.7 Gy of X-irradiation at weekly intervals, 23/23 (100%) of the p53 +/- females died of tumors within 36 weeks. In these animals, mammary adenocarcinoma (15/23, 65%), instead of lymphoma (7/23, 30%), was dominant. None of the similarly treated p53 +/+ females developed malignant tumors during the period. Mammary adenocarcinomas generated in p53 +/- females exposed or non-exposed to radiation showed a frequent loss of the p53 wild-type allele. Hence, we provided a useful experimental system to study radiation-induced mammary tumors in mice.
引用
收藏
页码:249 / 254
页数:6
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