Hydrolysis of p-nitrophenyl laurate in supercritical carbon dioxide:: comparison of two different enzymes

被引：4

作者：

Srivastava, S ^{[1
]}

Madras, G ^{[1
]}

机构：

[1] Indian Inst Sci, Dept Chem Engn, Bangalore 560012, Karnataka, India

来源：

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY | 2001年 / 76卷 / 08期

关键词：

enzyme-catalysed hydrolysis; hog pancreas lipase; P roqueforti; supercritical carbon dioxide;

D O I：

10.1002/jctb.442

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The enzymatic hydrolysis of p-nitrophenyl laurate (PNPL) to p-nitrophenol (PNP) was studied in supercritical carbon dioxide using crude Hog Pancreas Lipase (HPL) and P roqueforti Lipase (PR-L). The two enzymes were compared for their optimal activity temperatures and reaction kinetics. HPL had a higher optimal temperature (65 degreesC) and better yield (22.5%) compared with PRL whose optimal temperature was 50 degreesC with a 15.3% yield. This is considerably higher than the optimum temperature of 37 degreesC and yields of less than 1% observed in the aqueous medium. (C) 2001 Society of Chemical Industry.

引用

页码：890 / 892

页数：3