Hydrolysis of p-nitrophenyl laurate in supercritical carbon dioxide:: comparison of two different enzymes

被引：4
作者：
Srivastava, S [1 ]
Madras, G [1 ]
机构：
[1] Indian Inst Sci, Dept Chem Engn, Bangalore 560012, Karnataka, India
来源：
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY | 2001年 / 76卷 / 08期
关键词：
enzyme-catalysed hydrolysis; hog pancreas lipase; P roqueforti; supercritical carbon dioxide;
D O I：
10.1002/jctb.442
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The enzymatic hydrolysis of p-nitrophenyl laurate (PNPL) to p-nitrophenol (PNP) was studied in supercritical carbon dioxide using crude Hog Pancreas Lipase (HPL) and P roqueforti Lipase (PR-L). The two enzymes were compared for their optimal activity temperatures and reaction kinetics. HPL had a higher optimal temperature (65 degreesC) and better yield (22.5%) compared with PRL whose optimal temperature was 50 degreesC with a 15.3% yield. This is considerably higher than the optimum temperature of 37 degreesC and yields of less than 1% observed in the aqueous medium. (C) 2001 Society of Chemical Industry.
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页码：890 / 892
页数：3
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