Clinical applications of chemiluminescence

被引：218

作者：

Kricka, LJ ^{[1
]}

机构：

[1] Univ Penn, Dept Pathol & Lab Med, Philadelphia, PA 19104 USA

来源：

ANALYTICA CHIMICA ACTA | 2003年 / 500卷 / 1-2期

关键词：

chemiluminescence; immunoassay; nucleic acid assay; dioxetane; luminol; acridinium ester;

D O I：

10.1016/S0003-2670(03)00809-2

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This article reviews the clinical applications of chemiluminescence in routine testing and surveys the diverse applications of chemiluminescence in clinical research. In routine clinical testing, chemiluminescent labels (acridinium ester, acridinium sulfonamide) and detection reactions for peroxidase and alkaline phosphatase enzyme labels (luminol and adamantyl 1,2-dioxetane-based reactions, respectively) are widely used in immunoassay and nucleic acid probe assays (e.g. hybridization protection assay, Hybrid Capture assay). In clinical research the sensitivity, dynamic range and diversity of chemiluminescent assays has led to a vast range of applications, notably in protein and nucleic acid blotting, microarray-based assays, monitoring reactive oxygen species, and as detection reactions for substances separated by HPLC, capillary electrophoresis (CE), and flow-injection analysis. (C) 2003 Elsevier B.V All rights reserved.

引用

页码：279 / 286

页数：8

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