CREPT/RPRD1B associates with Aurora B to regulate Cyclin B1 expression for accelerating the G2/M transition in gastric cancer

被引：53

作者：

Ding, Lidan ^{[1
]}

Yang, Liu ^{[1
]}

He, Yuqi ^{[2
]}

Zhu, Bingtao ^{[1
]}

Ren, Fangli ^{[1
]}

Fan, Xuanzi ^{[1
]}

Wang, Yinyin ^{[1
]}

Li, Mengdi ^{[1
]}

Li, Jun ^{[3
]}

Kuang, Yanshen ^{[4
]}

Liu, Sihan ^{[1
]}

Zhai, Wanli ^{[1
]}

Ma, Danhui ^{[1
]}

Ju, Yanfang ^{[4
]}

Liu, Quentin ^{[5
]}

Jia, Baoqing ^{[4
]}

Sheng, Jianqiu ^{[2
]}

Chang, Zhijie ^{[1
]}

机构：

[1] Tsinghua Univ, Sch Med, State Key Lab Membrane Biol, Beijing 100084, Peoples R China

[2] PLA Army Gen Hosp, Dept Gastroenterol, Beijing 100700, Peoples R China

[3] Third Mil Med Univ, PLA, Inst Immunol, Chongqing 400038, Peoples R China

[4] Chinese Peoples Liberat Army Gen Hosp, Dept Gen Surg Pathol, Beijing 100853, Peoples R China

[5] Sun Yat Sen Univ, State Key Lab Canc South China, Canc Ctr, Guangzhou 510275, Guangdong, Peoples R China

来源：

CELL DEATH & DISEASE | 2018年 / 9卷

基金：

中国国家自然科学基金;

关键词：

CELL-CYCLE; CDK INHIBITORS; PROLIFERATION; GROWTH; TRANSCRIPTION; INCREASES; KINASES; P53;

D O I：

10.1038/s41419-018-1211-8

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Gastric cancer, like most of other cancers, has an uncontrolled cell cycle regulated by cyclins and cyclin-dependent kinases (CDKs). In this study, we reported that gastric cancer cells showed an accelerated G2/M transition promoted by CREPT/RPRD1B and Aurora kinase B (Aurora B). We found that CREPT/RPRD1B and Aurora B were coordinately expressed during the cell cycle in gastric cancer cells. Deletion of CREPT/RPRD1B disturbed the cell progression and extended the length of cell cycle, leading to a significant accumulation of mitotic cells. Mechanistically, we revealed that CREPT/RPRD1B interacted with Aurora B to regulate the expression of Cyclin B1 in gastric cancer cells. Interestingly, Aurora B phosphorylates S145 in a well-conserved motif of CREPT/RPRD1B. We proposed that phosphorylation of CREPT/RPRD1B by Aurora B is required for promoting the transcription of Cyclin B1, which is critical for the regulation of gastric tumorigenesis. Our study provides a mechanism by which gastric tumor cells maintain their high proliferation rate via coordination of Aurora B and CREPT/RPRD1B on the expression of Cyclin B1. Targeting the interaction of Aurora B and CREPT/RPRD1B might be a strategy for anti-gastric cancer therapy in the future.

引用

页数：15

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