Proofreading and aminoacylation of tRNAs before export from the nucleus

被引：265

作者：

Lund, E ^{[1
]}

Dahlberg, JE ^{[1
]}

机构：

[1] Univ Wisconsin, Dept Biomol Chem, Madison, WI 53706 USA

来源：

SCIENCE | 1998年 / 282卷 / 5396期

关键词：

D O I：

10.1126/science.282.5396.2082

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

After synthesis and processing in the nucleus, mature transfer RNAs (tRNAs) are exported to the cytoplasm in a Ran.guanosine triphosphate-dependent manner. Export of defective or immature tRNAs is avoided by monitoring both structure and function of tRNAs in the nucleus, and only tRNAs with mature 5' and 3' ends are exported. ALL tRNAs examined can be aminoacylated in nuclei of Xenopus oocytes, thereby providing a possible mechanism for functional proofreading of newly made tRNAs. Inhibition of aminoacylation of a specific tRNA retards its appearance in the cytoplasm, indicating that nuclear aminoacylation promotes efficient export.

引用

页码：2082 / 2085

页数：4

共 45 条

[11]

DREHER TW, IN PRESS J BIOL CHEM

[12] Protein kinesis - Nucleocytoplasmic transport [J].

Gorlich, D ;

Mattaj, IW .

SCIENCE, 1996, 271 (5255) :1513-1518

[13] Yeast Los1p has properties of an exportin-like nucleocytoplasmic transport factor for tRNA [J].

Hellmuth, K ;

Lau, DM ;

Bischoff, FR ;

Künzler, M ;

Hurt, E ;

Simos, G .

MOLECULAR AND CELLULAR BIOLOGY, 1998, 18 (11) :6374-6386

[14] YEAST MUTANT WHICH ACCUMULATES PRECURSOR TRANSFER-RNAS [J].

HOPPER, AK ;

BANKS, F ;

EVANGELIDIS, V .

CELL, 1978, 14 (02) :211-219

[15] PROCESSING OF INTERVENING SEQUENCES - NEW YEAST MUTANT WHICH FAILS TO EXCISE INTERVENING SEQUENCES FROM PRECURSOR TRANSFER-RNAS [J].

HOPPER, AK ;

SCHULTZ, LD ;

SHAPIRO, RA .

CELL, 1980, 19 (03) :741-751

[16] CLONING AND CHARACTERIZATION OF LOS1, A SACCHAROMYCES-CEREVISIAE GENE THAT AFFECTS TRANSFER-RNA SPLICING [J].

HURT, DJ ;

WANG, SS ;

LIN, YH ;

HOPPER, AK .

MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (03) :1208-1216

[17] The asymmetric distribution of the constituents of the Ran system is essential for transport into and out of the nucleus [J].

Izaurralde, E ;

Kutay, U ;

vonKobbe, C ;

Mattaj, IW ;

Gorlich, D .

EMBO JOURNAL, 1997, 16 (21) :6535-6547

[18] NUCLEAR EXPORT OF DIFFERENT CLASSES OF RNA IS MEDIATED BY SPECIFIC FACTORS [J].

JARMOLOWSKI, A ;

BOELENS, WC ;

IZAURRALDE, E ;

MATTAJ, IW .

JOURNAL OF CELL BIOLOGY, 1994, 124 (05) :627-635

[19] REGULATION OF RNA PROCESSING AND TRANSPORT BY A NUCLEAR GUANINE-NUCLEOTIDE RELEASE PROTEIN AND MEMBERS OF THE RAS SUPERFAMILY [J].

KADOWAKI, T ;

GOLDFARB, D ;

SPITZ, LM ;

TARTAKOFF, AM ;

OHNO, M .

EMBO JOURNAL, 1993, 12 (07) :2929-2937

[20] AMINOACYL-TRANSFER-RNA SYNTHETASES FROM HIGHER EUKARYOTES [J].

KISSELEV, LL ;

WOLFSON, AD .

PROGRESS IN NUCLEIC ACID RESEARCH AND MOLECULAR BIOLOGY, VOL 48, 1994, 48 :83-142

← 1 2 3 4 5 →