A highly sensitive red-emitting probe for the detection of viscosity changes in living cells, zebrafish, and human blood samples

被引：47

作者：

Fu, Manlin ^{[1
]}

Shen, Wei ^{[2
]}

Chen, Yajun ^{[1
]}

Yi, Wenjun ^{[1
]}

Cai, Chunhui ^{[1
]}

Zhu, Liquan ^{[1
]}

Zhu, Qing ^{[1
]}

机构：

[1] Zhejiang Univ Technol, Coll Biotechnol & Bioengn, Key Lab Bioorgan Synth Zhejiang Prov, Hangzhou 310014, Peoples R China

[2] Jinhua Cent Hosp, Dept Gen Surg, Jinhua 321000, Zhejiang, Peoples R China

来源：

JOURNAL OF MATERIALS CHEMISTRY B | 2020年 / 8卷 / 06期

基金：

中国国家自然科学基金;

关键词：

2-PHOTON FLUORESCENT-PROBE; MITOCHONDRIA; HYPERTENSION; SENSORS; DYES;

D O I：

10.1039/c9tb02023c

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

Intracellular viscosity can be measured to reflect the state of living cells. Fluorescent probes are powerful tools for viscosity detection in vivo. Herein, we report on a novel red-emitting viscosity-sensitive probe DJH with a large Stokes shift of 165 nm, showing a 400-fold fluorescence enhancement from PBS solution to 90% glycerol. The probe was suitable for the visualization of the changes in viscosity within living cells and also in zebrafish treated with microplastics for the first time. Furthermore, the viscosity of fresh blood from diabetic mice and hypertensive and diabetic patients was first evaluated by using DJH. These results showed that the probe has a wide range of potential applications in basic research on environmental pollution and in the pre-diagnosis of patients.

引用

页码：1310 / 1315

页数：6

共 47 条

[1] Gradual alteration of mitochondrial structure and function by β-amyloids:: Importance of membrane viscosity changes, energy deprivation, reactive oxygen species production, and cytochrome c release [J].