Purification and characterization of sucrose synthase isozymes from etiolated rice seedlings

被引：1

作者：

Huang, DY ^{[1
]}

Wang, AY ^{[1
]}

机构：

[1] Natl Taiwan Univ, Dept Agr Chem, Taipei 10764, Taiwan

来源：

BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL | 1998年 / 46卷 / 01期

关键词：

rice; sucrose synthase; isozymes; immunoprecipitation; purification;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Presence of homo- and hetero-tetrameric rice sucrose synthase (RSS) isoforms in etiolated rice seedlings was demonstrated by immunoprecipitation using monospecific antibodies. Three RSS isozymes with various pi values were purified by ammonium sulfate fractionation, Sepharose CL-6B gel filtration, DEAE-Sephacel and Mono-Q ion exchange chromatographies. They were characterized as heterotetramers composed of RSS1 and RSS2 subunits. All of them used UDP as the best substrate. The presence of divalent metal ions increased the activities of synthesis but inhibited the cleavage of sucrose.

引用

页码：107 / 113

页数：7

共 19 条

[1] A MEMBRANE-ASSOCIATED FORM OF SUCROSE SYNTHASE AND ITS POTENTIAL ROLE IN SYNTHESIS OF CELLULOSE AND CALLOSE IN PLANTS [J].