Synthesis and purification of the aflatoxin B1-lysine adduct

被引：13

作者：

Sass, Daiane Cristina ^{[1
]}

Jager, Alessandra Vincenzi ^{[1
]}

Tonin, Fernando Gustavo ^{[2
]}

Rosim, Roice Eliana ^{[1
]}

Constantino, Mauricio Gomes ^{[3
]}

Fernandes Oliveira, Carlos Augusto ^{[1
]}

机构：

[1] Univ Sao Paulo, Dept Food Engn, Sch Anim Sci & Food Engn, Pirassununga, SP, Brazil

[2] Univ Sao Paulo, Dept Biossyst Engn, Sch Anim Sci & Food Engn, Pirassununga, SP, Brazil

[3] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Dept Chem, BR-14049 Ribeirao Preto, SP, Brazil

来源：

TOXIN REVIEWS | 2015年 / 34卷 / 01期

基金：

巴西圣保罗研究基金会;

关键词：

AFB(1)-lysine; aflatoxins; biomarkers; synthesis; SERUM-ALBUMIN ADDUCT; PLASMA-ALBUMIN; B-1; EXPOSURE; BLOOD; LIVER; ASSOCIATION; BIOMARKERS; OXIDATION; PRODUCTS;

D O I：

10.3109/15569543.2014.994132

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

This work reports the chemical synthesis of aflatoxin B-1 (AFB(1))-lysine based on procedures available in the literature, but using lysine without a protection group in the a-amine group. AFB(1)-exo-8,9-epoxide was obtained by epoxidation of AFB(1) with chloroperoxybenzoic acid in dichloromethane and phosphate buffer. Purification and identification of the AFB(1)-lysine were conducted by liquid chromatography (LC), and its structure was confirmed by LC with mass spectrometer and diode-array detection. The preparation of AFB(1)-lysine using lysine without a protection group in the alpha-amine group was completed in 24 h, being a practical modification of available methods that can be reproduced in analytical laboratories.

引用

页码：53 / 59

页数：7

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