Volatile Compounds of Endophytic Bacillus spp. have Biocontrol Activity Against Sclerotinia sclerotiorum

被引:114
作者
Massawe, Venance Colman [1 ]
Hanif, Alvina [1 ]
Farzand, Ayaz [1 ]
Mburu, David Kibe [1 ]
Ochola, Sylvans Ochieng [1 ]
Wu, Liming [1 ]
Tahir, Hafiz Abdul Samad [1 ]
Gu, Qin [1 ]
Wu, Huijun [1 ]
Gao, Xuewen [1 ]
机构
[1] Nanjing Agr Univ, Minist Educ, Key Lab Integrated Management Crop Dis & Pests, Dept Plant Pathol,Coll Plant Protect, Nanjing, Jiangsu, Peoples R China
基金
中国国家自然科学基金;
关键词
INDUCED SYSTEMIC RESISTANCE; OXALIC-ACID; RHIZOBACTERIAL VOLATILES; BACTERIAL VOLATILES; GROWTH; FUNGI; IDENTIFICATION; ELICITOR; ALCOHOLS; SIGNALS;
D O I
10.1094/PHYTO-04-18-0118-R
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
To develop an effective biological agent to control Sclerotinia sclerotiorum, three endophytic Bacillus spp. strains with high antagonistic activity were isolated from maize seed and characterized. In vitro assays revealed that the Bacillus endophytes could produce volatile organic compounds (VOC) that reduced sclerotial production and inhibited mycelial growth of S. sclerotiorum. Gas chromatography-mass spectrometry revealed that the selected strains produced 16 detectable VOC. Eight of the produced VOC exhibited negative effects on S. sclerotiorum, while a further four induced accumulation of reactive oxygen species in mycelial cells. A mixture of VOC produced by Bacillus velezensis VM11 caused morphological changes in the ultrastructure and organelle membranes of S. sclerotiorum mycelial cells. The bromophenol blue assay revealed a yellow color of untreated fungal mycelium, which grew faster and deeper from 24 to 72 h postinoculation, as an indication of reduced pH. The potassium permanganate (KMnO4) titration assay showed that the rate of oxalic acid accumulation was higher in minimal salt liquid medium cultures inoculated with untreated fungal plugs compared with the Bacillus VOC-treated ones. Interestingly, biological control assays using host-plant leaves challenged with treated fungal mycelial plugs produced reduced lesions compared with the control. These findings provide new viable possibilities of controlling diseases caused by S. sclerotiorum using VOC produced by Bacillus endophytes.
引用
收藏
页码:1373 / 1385
页数:13
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