Phase diagram determination for phospholipid/sterol membranes using deuterium NMR

被引:20
作者
Hsueh, YW
Zuckermann, M
Thewalt, J [1 ]
机构
[1] Simon Fraser Univ, Dept Phys, Burnaby, BC V5A 1S6, Canada
[2] Natl Cent Univ, Dept Phys, Chungli 320, Taiwan
[3] Simon Fraser Univ, Dept Mol Biol & Biochem, Burnaby, BC V5A 1S6, Canada
关键词
phase diagram; phosphatidylcholine; ergosterol; H-2; NMR;
D O I
10.1002/cmr.a.20034
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Deuterium NMR is used to investigate the phase behavior of membranes containing ergosterol and to measure the temperature-ergosterol mole fraction phase diagram. In particular, our approach to analyzing the NMR spectra has allowed us to definitively locate and characterize the region where two liquid crystalline phases coexist. The general methodology for phase boundary determination in phospholipid/sterol membranes is presented. (c) 2005 Wiley Periodicals, Inc.
引用
收藏
页码:35 / 46
页数:12
相关论文
共 16 条
[1]   Functions of lipid rafts in biological membranes [J].
Brown, DA ;
London, E .
ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 1998, 14 :111-136
[2]   A contribution to the theory of permeability of thin films [J].
Danielli, JF ;
Davson, H .
JOURNAL OF CELLULAR AND COMPARATIVE PHYSIOLOGY, 1935, 5 (04) :495-508
[3]   THE DESCRIPTION OF MEMBRANE LIPID CONFORMATION, ORDER AND DYNAMICS BY H-2-NMR [J].
DAVIS, JH .
BIOCHIMICA ET BIOPHYSICA ACTA, 1983, 737 (01) :117-171
[4]   QUADRUPOLAR ECHO DEUTERON MAGNETIC-RESONANCE SPECTROSCOPY IN ORDERED HYDROCARBON CHAINS [J].
DAVIS, JH ;
JEFFREY, KR ;
BLOOM, M ;
VALIC, MI ;
HIGGS, TP .
CHEMICAL PHYSICS LETTERS, 1976, 42 (02) :390-394
[5]   On bimolecular layers of lipoids on the chromocytes of the blood. [J].
Gorter, E ;
Grendel, F .
JOURNAL OF EXPERIMENTAL MEDICINE, 1925, 41 (04) :439-443
[6]  
HSUEH YW, IN PRESS BIOPHYS J
[7]  
IPSEN JH, 1987, BIOCHIM BIOPHYS ACTA, V905, P162
[8]   SMOOTHED ORIENTATIONAL ORDER PROFILE OF LIPID BILAYERS BY H-2-NUCLEAR MAGNETIC-RESONANCE [J].
LAFLEUR, M ;
FINE, B ;
STERNIN, E ;
CULLIS, PR ;
BLOOM, M .
BIOPHYSICAL JOURNAL, 1989, 56 (05) :1037-1041
[10]   Role of cholesterol in lipid raft formation: lessons from lipid model systems [J].
Silvius, JR .
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 2003, 1610 (02) :174-183