Enzymatic production of isoamyl acetate in an ionic liquid-alcohol biphasic system

被引：47

作者：

Feher, E. ^{[1
]}

Illeova, V. ^{[2
]}

Kelemen-Horvath, I. ^{[1
]}

Belafi-Bako, K. ^{[1
]}

Polakovic, M. ^{[2
]}

Gubicza, L. ^{[1
]}

机构：

[1] Univ Pannonia, Res Inst Chem & Proc Engn, H-8200 Veszprem, Hungary

[2] Slovak Tech Univ, Fac Chem & Food Technol, Dept Chem & Biochem Engn, Bratislava 81237, Slovakia

来源：

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC | 2008年 / 50卷 / 01期

关键词：

biphasic system; flavour; isoamyl acetate; ionic liquid; Candida antarctica lipase B;

D O I：

10.1016/j.molcatb.2007.09.019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A systematic investigation and optimization on the conditions for the biphasic enzymatic production of isoamyl acetate in ionic liquid solvents is reported. As a catalyst, immobilized Candida antarctica lipase B was used. As the best imidazolium type ionic liquid, [bmim]PF6 was selected and other reaction conditions such as initial water content, acetic acid to isoamyl alcohol substrate ratio, [bmim]PF6 to isoamyl alcohol solvent ratio, enzyme amount and temperature were optimized. The recyclability of the applied IL per se and together with enzyme were also studied and found to be reusable in 7 and 10 cycles, respectively. With the adjusted optimum conditions high isoamyl acetate yields (near 100%) were achieved. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：28 / 32

页数：5

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